猪囊尾蚴西昌分离株线粒体cox1和nad1基因的序列测定与种系发育分析

应用PCR技术对3个猪囊尾蚴西昌分离株的线粒体细胞色素C氧化酶第Ⅰ亚基(cox1)基因全序列和烟酰胺腺嘌呤二核苷酸还原酶第一亚基(nad1)基因部分序列进行扩增,分析其遗传变异,并用Mega 5.0程序NJ法绘制种系发育树,探讨不同地区来源的猪囊尾蚴种系发育关系。测序结果显示,猪囊尾蚴的cox1基因全序列长度为1 620bp,nad1基因部分序列长度为796bp。cox1和nad1基因序列均存在一定的遗传变异,且cox1基因序列的变异高于nad1序列。种系发育分析结果显示,所有猪囊尾蚴分离株构成一个分支,3个西昌分离株均属于猪带绦虫亚洲基因型。结果表明,cox1和nad1基因均可用于猪带绦虫的分子分类,为猪带绦虫病/猪囊尾蚴病的分子诊断奠定了基础。

Sequencing and Phylogenetic Analysis of Mitochondrial cox1 and nad1 Genes for Cysticercus Cellulosae in Xichang

The complete sequences of mitochondrial cytochrome C oxidase subunitⅠ(cox1) gene and the partial sequences of NADH dehydrogenase subunit 1 (nad1) gene of three cysticercus cellulosae samples isolated from Xichang were amplified by PCR, and sequenced to analyze their genetic variations, NJ trees were reconstructed using the Mega version 5.0 software.The results showed that the complete sequence of cox1 gene and partial sequence of nad1 gene of cysticercus cellulosae were 1 620 and 796 bp, respectively.There were some genetic variations in cox1 and nad1 genes within cysticercus cellulosae, the level of variation was higher in the cox1 gene than in the nad1 gene.The phylogenetic trees showed that all the cysticercus cellulosae isolates were formed a single cluster, and the three Xichang isolates belonged to Asian genotype.The results indicated that cox1 and nad1 genes could be used in molecular classification of Taenia solium, and established the foundation for molecular diagnosis of taeniasis/cysticercosis.