首页 | 本学科首页   官方微博 | 高级检索  
     检索      

冷应激对湖羊血清因子及热休克蛋白70 mRNA表达的影响
引用本文:杨莉,张莉,齐亚银,沈文,李岩,张鲁安.冷应激对湖羊血清因子及热休克蛋白70 mRNA表达的影响[J].中国畜牧兽医,2015,42(4):890-895.
作者姓名:杨莉  张莉  齐亚银  沈文  李岩  张鲁安
作者单位:1. 石河子大学动物科技学院, 石河子 832003;2. 新疆生产建设兵团畜牧兽医工作总站, 乌鲁木齐 830063
基金项目:石河子大学优秀青年基金项目(2012ZRKXYQ02)
摘    要:为研究冷应激对湖羊免疫系统及热休克蛋白70(heat stress proteins 70,Hsp70)的影响,试验分别采用实时荧光定量PCR和ELISA方法检测冷应激前后湖羊肝脏、肺脏、脾脏、淋巴结组织中Hsp70mRNA表达量及血清中白细胞介素-2(IL-2)、白细胞介素-4(IL-4)浓度。结果显示,与冷应激前相比,冷应激后湖羊肝脏、肺脏及脾脏组织中Hsp70mRNA表达量均极显著增加(P0.01),其中肝脏的表达量尤为显著;冷应激后湖羊血清中IL-2、IL-4的浓度均呈下降趋势,其中IL-4浓度下降尤为显著(P0.01)。结果表明,冷应激条件下湖羊各组织中Hsp70mRNA表达增强,可提高动物机体的自我保护机能,增强对外界不良刺激的抵抗力,但细胞因子IL-2和IL-4的浓度下降,表明冷应激抑制机体免疫系统。

关 键 词:冷应激  热休克蛋白70  白细胞介素-2  白细胞介素-4  湖羊  
收稿时间:2014-09-14

Effect of Cold Stress on Serum Factor and Hsp70 mRNA in Hu Sheep
YANG Li,ZHANG Li,QI Ya-yin,SHEN Wen,LI Yan,ZHANG Lu-an.Effect of Cold Stress on Serum Factor and Hsp70 mRNA in Hu Sheep[J].China Animal Husbandry & Veterinary Medicine,2015,42(4):890-895.
Authors:YANG Li  ZHANG Li  QI Ya-yin  SHEN Wen  LI Yan  ZHANG Lu-an
Institution:1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;2. The Xinjiang Production and Construction Corps of Animal Husbandry and Veterinary Station, Urumqi 830063, China
Abstract:This experiment was to study the effect of cold stress on the immune system and heat stress proteins 70 (Hsp70) in Hu sheep. Using Real-time fluorescence quantitative PCR and ELISA, the expression quantity of Hsp70 mRNA in Hu sheep''s liver, lung, spleen, lymph node tissue and concentrations of IL-4, IL-2 in serum were detected respectively before and after cold stress. The results showed that the Hsp70 mRNA expression quantity in liver, lung and spleen tissues of Hu sheep was extremely significantly increased after cold stress compared with before (P<0.01), and the expression of the liver was particularly significant. After cold stress, the IL-2, IL-4 concentrations in Hu sheep serum showed a decreasing trend, especially IL-4 concentration decreased extremely significantly (P<0.01). The results indicated that enhanced expression of Hsp70 mRNA after cold stress in Hu sheep tissues could improve the animal''s function of self-protection and enhance resistance to adverse external stimulate, but the decreases in cytokines IL-2 and IL-4 concentrations indicated that cold stress suppressed the body''s immune system.
Keywords:cold stress  Hsp70  IL-2  IL-4  Hu sheep
本文献已被 CNKI 等数据库收录!
点击此处可从《中国畜牧兽医》浏览原始摘要信息
点击此处可从《中国畜牧兽医》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号