冷应激对湖羊血清因子及热休克蛋白70 mRNA表达的影响

为研究冷应激对湖羊免疫系统及热休克蛋白70(heat stress proteins 70,Hsp70)的影响,试验分别采用实时荧光定量PCR和ELISA方法检测冷应激前后湖羊肝脏、肺脏、脾脏、淋巴结组织中Hsp70mRNA表达量及血清中白细胞介素-2(IL-2)、白细胞介素-4(IL-4)浓度。结果显示,与冷应激前相比,冷应激后湖羊肝脏、肺脏及脾脏组织中Hsp70mRNA表达量均极显著增加(P0.01),其中肝脏的表达量尤为显著;冷应激后湖羊血清中IL-2、IL-4的浓度均呈下降趋势,其中IL-4浓度下降尤为显著(P0.01)。结果表明,冷应激条件下湖羊各组织中Hsp70mRNA表达增强,可提高动物机体的自我保护机能,增强对外界不良刺激的抵抗力,但细胞因子IL-2和IL-4的浓度下降,表明冷应激抑制机体免疫系统。

Effect of Cold Stress on Serum Factor and Hsp70 mRNA in Hu Sheep

This experiment was to study the effect of cold stress on the immune system and heat stress proteins 70 (Hsp70) in Hu sheep. Using Real-time fluorescence quantitative PCR and ELISA, the expression quantity of Hsp70 mRNA in Hu sheep''s liver, lung, spleen, lymph node tissue and concentrations of IL-4, IL-2 in serum were detected respectively before and after cold stress. The results showed that the Hsp70 mRNA expression quantity in liver, lung and spleen tissues of Hu sheep was extremely significantly increased after cold stress compared with before (P<0.01), and the expression of the liver was particularly significant. After cold stress, the IL-2, IL-4 concentrations in Hu sheep serum showed a decreasing trend, especially IL-4 concentration decreased extremely significantly (P<0.01). The results indicated that enhanced expression of Hsp70 mRNA after cold stress in Hu sheep tissues could improve the animal''s function of self-protection and enhance resistance to adverse external stimulate, but the decreases in cytokines IL-2 and IL-4 concentrations indicated that cold stress suppressed the body''s immune system.