扭角羚肺炎克雷伯氏菌的分离鉴定

本试验旨在报告四川省野生扭角羚自然生存过程中致病性细菌的检测及生物学特征。试验对发病死亡扭角羚病变组织进行细菌学检查及分离,对分别从肝脏、脾脏和肺脏分离得到的4株优势菌进行形态特征、生化特性和16SrDNA分子鉴定,判定为肺炎克雷伯氏菌。这4株菌对供试小白鼠均有致病性。测其LD50,分别为2.9×107、2.9×107、4.5×107和1.1×108 CFU/只。4株分离菌对供试的先锋霉素等敏感,对阿米卡星等中度敏感,对红霉素等耐药。推测这4株肺炎克雷伯氏菌是导致扭角羚死亡的主要病原菌。

Isolation and Identification of K.pneumoniae in Takin

In order to report the detection and main biological characteristics of pathogenic bacteria isolated from wild takin in the process of the natural survival, four strains of the predominant bacteria from pathologic tissues of dead takin''s livers, spleens and lungs were examined and isolated.The detections of biochemical characteristics and 16S rDNA gene were conducted.The strains were isolated and identified as K.pneumoniae.The four isolates had pathogenicity to mice.LD₅₀ of the four isolates were 2.9×10⁷, 2.9×10⁷, 4.5×10⁷ and 1.1×10⁸ CFU, respectively.The antibiotic sensitivity test revealed that the strains were sensitive to cephalothin and so on, moderate sensitive to amikacin and so on, and resistant to erythrocin and so on.The four strains of K.pneumoniae were the primary bacterial pathogen leading to the death of takin.