利巴韦林对猫细小病毒感染诱导F81猫肾细胞中凋亡相关基因表达的影响

试验将F81猫肾细胞随机分为对照组、药物组(猫细小病毒(FPV)+利巴韦林)、病毒组(FPV感染),采用实时荧光定量PCR的方法,检测Bcl-2、Bcl-xl、Bax和Caspase 3等凋亡相关基因的相对表达量。结果显示,与对照组相比,病毒组细胞凋亡增加,抗凋亡基因Bcl-2 mRNA表达受到抑制,Bax mRNA和 Caspase 3 mRNA的相对表达量均有不同程度的上调。药物组细胞的抗凋亡基因Bcl-2和Bcl-xl的表达水平显著高于病毒组(P<0.05),且总体呈上调状态,但72 h时Bcl-xl表达量减少;而药物组的促凋亡基因Bax和Caspase 3的表达量显著低于病毒组(P<0.05),48 h前,Bax 的表达量呈递减状态,72 h时检测结果显示药物组Bax表达量相比对照组显著上调(P<0.05),但仍显著低于病毒组(P<0.05);与对照组相比,病毒组和药物组Caspase 3的表达量在72 h时显著上调(P<0.05)。综上所述,利巴韦林可在基因水平通过上调抗凋亡基因Bcl-2和Bcl-xl和下调促凋亡基因Bax和Caspase 3的表达来抑制FPV感染诱导的F81猫肾细胞凋亡发生。

Effects of Ribavirin on Expression of Apoptotic Related Genes of F81 Cells Infected by Feline Panleukopenia Virus

The F81 cells were divided into three groups,control group,feline panleukopenia virus (FPV) infected group and ribavirin treatment group,we used Real-time fluorescent quanlitative PCR to detect the expression of Bcl-2,Bcl-xl,Bax and Caspase 3.Real-time fluorescent quanlitative PCR results showed that the number of apoptotic cells increased in FPV infected group compared with the control group,and mRNA levels of Bcl-2 was inhibited,which was an anti-apoptotic gene,while the expressions of Bax and Caspase 3 were both increased.In ribavirin treatment group,expression levels of Bcl-2 and Bcl-xl were significantly higher than that of FPV infected group (P<0.05),and had a peak at 48 h in all time points.However,mRNA levels of Bax and Caspase 3 in ribavirin treatment group were significantly lower than that of FPV infected group (P<0.05),and the lowest levels were at 72 h in all time points.In a word,ribavirin could inhibit the apoptosis of F81 cells infected by FPV at the genetic level.