小尾寒羊线粒体基因组遗传变异分析

本试验旨在利用基因组扫描策略和DNA混池测序技术分析小尾寒羊线粒体基因组遗传变异.结果表明,小尾寒羊线粒体基因组编码区共有95个变异位点,多肽编码区除ATPase8基因无突变外,其余12个多肽编码基因均有突变位点,变异位点总数为83个,错义突变为19个,分别为:T3544A、T4209C、C7501A、A8040G、G8265C、A9376G、C9975T、G10119A、G10938A、G11046A、G12571C、G13041A、C13576T、T13588C、C13777T、C13789T、T13837C、T13855C、A13876G.12S rRNA区域有3个变异位点,分别为:T281C、C291T、A538G;16S rRNA区域有5个变异位点,分别为:A1099T、T1112C、T2200C、C2444T、T2635C;tRNA区域有4个突变位点,分别为:tRNA-Tyr (G5295A)、tRNA-Lys (T7720G)、tRNA-His (C11607T)、tRNA-Ser (G11669A).由此可知,小尾寒羊线粒体基因组编码区多态性较丰富,该试验结果为核外遗传效应研究提供了分子生物学基础.

Genetic Variation Analysis of Mitochondrial Genome in Small-tailed Han Sheep

Mitochondrial genome variations of Small-tailed Han sheep were analyzed through sequencing of DNA pooling and genome scanning in this experiment.The results showed that the regions coding for protein-tRNA-rRNA contained 95 variated sites, 83 mutations were found in coding for 12 polypeptides regions excepted ATPase8 gene, 19 missense mutations included T3544A, T4209C, C7501A, A8040G, G8265C, A9376G, C9975T, G10119A, G10938A, G11046A, G12571C, G13041A, C13576T, T13588C, C13777T, C13789T, T13837C, T13855C and A13876G, 3 variations (T281C, C291T and A538G) were in 12S rRNA gene, and 5 substituted sites (A1099T, T1112C, T2200C, C2444T and T2635C) were in 16S rRNA gene.The tRNA gene contained 4 mutations included G5295A in tRNA-Tyr, T7720G in tRNA-Lys, C11607T in tRNA-His, G11669A in tRNA-Ser.Therefore, polymorphism of mtDNA regions coding for protein-tRNA-rRNA was abundant in Small-tailed Han sheep, and the results provided molecular biological foundation for study on extracellular heredity effects.