Kiss-1基因在不同发育阶段五指山猪睾丸中的表达及定位研究

试验采用荧光定量技术研究亲吻素-1(Kiss-1)基因mRNA在30、60、80(初情期)和120日龄五指山公猪睾丸中的表达情况,并采用免疫组化技术定位其表达。结果显示,Kiss-1mRNA在五指山猪睾丸中的表达随着年龄的增长表达量逐渐升高,到初情期(80日龄)时达到最高(P0.05),随后到120日龄时显著降低(P0.05);免疫组化结果显示,睾丸间质细胞、精原细胞、初级精母细胞、次级精母细胞、圆形精子细胞及间质细胞中均发现Kiss-1阳性反应产物,而在精子中未发现Kiss-1阳性表达产物。结果证实,Kiss-1基因在五指山猪精子发生过程中发挥着重要作用。

Study on Expression and Localization of Kiss-1 Gene in Different Developmental Stages of Wuzhishan Pig Testis

The research aimed to study the expression of Kiss-1 in different developmental stages of Wuzhishan pig testis.The expression of Kiss-1 mRNA in 30,60,80 and 120 day-old Wuzhishan pig testis were studied using Real-time PCR.Cellular localization of Kiss-1 in testis was examined by immunohistochemistry.The results showed that the expression of Kiss-1 mRNA was increased with the age before puberty (P<0.05),and reached the highest level in 80-day-old testis (P<0.05),then decreased at 120 days of age (P<0.05).There was a positive expression of Kiss-1 in interstitial tissue,spermiogonium,primary spermatocyte,second spermatocyte,and round spermatid of adult Wuzhishan pig testis.However,the Kiss-1 immunopositive production was not found in sperm.The results confirmed that Kiss-1 gene played important role in spermatogenesis of Wuzhishan pig.