我国部分蓖麻品种遗传资源SSR分析及DNA指纹图谱

为了解蓖麻品种间的遗传多样性、构建指纹图谱,利用171对SSR及EST-SSR引物对30份国内蓖麻品种和1份法国品种进行遗传多样性和亲缘关系分析。结果表明蓖麻品种间呈中度多态性,平均每位点有2.267个等位基因数,香农指数、期望杂合度和多态性信息含量(PIC)分别为0.553、0.347和0.289。聚类分析显示在相似系数0.730处将31份蓖麻品种分为7个类群,来源于相同育种单位或相同省区的大部分品种聚在一起。另外,利用10对扩增清晰、多态性好的引物构建了31份蓖麻品种的指纹图谱,可用于品种鉴别。

Genetic diversity and DNA fingerprints of castor bean cultivars based on SSR markers

 To investigate the genetic diversity and phylogenetic relationship of castor, genetic diversities and relationships among 30 domestic cultivars and 1 French cultivar were assessed by SSR and EST-SSR markers. Results showed a moderate diversity level among these cultivars, with average 2.267 alleles per locus, 0.553 Shannon index, 0.345 Nei''s expected heterozygosity and 0.289 polymorphism information content (PIC) respectively. The 31 castor cultivars were clustered into 7 groups at the similarity coefficient of 0.730. Most cultivars were clustered into the same breeding units or regions. Among the 171 primers, 10 were used to construct a clear and polymorphic footprint，and can be used in the identification of cultivars.