| 应用PCR方法检测蜡样芽胞杆菌的研究 |
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| 引用本文： | 王振国,刘和平,刘金华,蔡阳,肖成蕊,罗雁菲,徐宝梁.应用PCR方法检测蜡样芽胞杆菌的研究[J].吉林农业大学学报,2006,28(6):671-673. |
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| 作者姓名： | 王振国 刘和平 刘金华 蔡阳 肖成蕊 罗雁菲 徐宝梁 |
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| 作者单位： | 1. 吉林出入境检验检疫局技术中心,长春,130062
2. 中国检验检疫科学研究院,北京,100025 |
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| 摘 要： | 采用PCR技术,通过扩增蜡样芽胞杆菌的gyrB基因来实现对蜡样芽胞杆菌的快速检测。结果表明,以gyrB为目的基因设计引物建立的PCR检测方法能特异性地扩增出蜡样芽胞杆菌,且与其他芽胞杆菌菌株均无交叉反应,其检测敏感性可达9 cfu/mL,能够应用于蜡样芽胞杆菌的鉴定。
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| 关 键 词： | 蜡样芽胞杆菌 gyrB基因 PCR检测 |
| 文章编号： | 1000-5684（2006）06-0671-03 |
| 收稿时间： | 2006-02-08 |
| 修稿时间： | 2006-02-082006-05-26 |
Identification of Bacillus cereus by Gyrase B gene with PCR Method |
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| WANG Zhen-guo, LIU He-ping, LIU Jin-hua, CAI Yang, XIAO Cheng-rui,et al..Identification of Bacillus cereus by Gyrase B gene with PCR Method[J].Journal of Jilin Agricultural University,2006,28(6):671-673. |
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| Authors: | WANG Zhen-guo LIU He-ping LIU Jin-hua CAI Yang XIAO Cheng-rui |
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| Institution: | 1. Center of Technology, Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China ; 2. Chinese Academy of Inspection and Quarantine, Beijing 100025, China |
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| Abstract: | Bacillus cereus is an important species of bacillus genus.It has close relationship in taxonomy with other Bacillus.Commonly,it is difficult to differentiate it from other Bacillus species.In this study,a PCR method for rapid and specific detection of Bacillus cereus based on the gyr B gene sequences is developed.The result shows that amplification gyr B gene by the PCR method allows faster detection of Bacillus cereus,its detection limit is 9 cfu/mL.This method can be used in healthy food control and Bacillus cereus identification. |
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| Keywords: | Bacillus cereus gyr B gene PCR detection |
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