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| 巴氏杆菌对链霉素和磺胺耐药机制的研究 | |
| 引用本文： | 金天明,高丰,佟庆彬,武迎红.巴氏杆菌对链霉素和磺胺耐药机制的研究[J].畜牧与兽医,2007,39(2):5-7. |
| 作者姓名： | 金天明 高丰 佟庆彬 武迎红 |
| 作者单位： | 1. 天津农学院动物科学系,天津,300384 2. 吉林大学畜牧兽医学院,吉林,长春,130062 3. 吉林市江南公园,吉林,吉林,13200 4. 内蒙古民族大学动物科技学院,内蒙古,通辽,028000 |
| 基金项目： | 国家自然科学基金资助项目（30460103）. |
| 摘 要： | 根据巴氏杆菌的链霉素耐药基因StrA和磺胺耐药基因SulⅡ序列设计2对引物,以临床分离的4株禽源巴氏杆菌耐药株为模板,扩增出StrA、SulⅡ基因序列。经克隆和核苷酸序列测定,克隆的基因与文献报道的耐药基因序列(NC-001774)有较高的同源性(≥98.9%);试验结果显示,耐药株巴氏杆菌对链霉素和磺胺耐药性的差异与StrA、SulⅡ基因突变有关(耐药性高的菌株基因突变率高)。试验中所有耐药菌均在StrA基因编码的第204位氨基酸处存在突变;所有耐药菌均在SulⅡ基因编码的第159,246,384,584,590,591,597,582位氨基酸处存在突变,上述突变基因可能是导致巴氏杆菌对链霉素和磺胺产生耐药性的主要因素。 |
| 关 键 词： | 巴氏杆菌 StrA基因 SulⅡ基因 基因突变 耐药机制 |
| 文章编号： | 0529-5130（2007）02-0005-03 |
| 修稿时间： | 2006-04-07 |
The mechanism of drug resistance of Streptomycin and sulfonamides in Pastuerella Multocida |
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| JIN Tian-ming,GAO Feng,TONG Qing-bin,WU Ying-hong.The mechanism of drug resistance of Streptomycin and sulfonamides in Pastuerella Multocida[J].Animal Husbandry & Veterinary Medicine,2007,39(2):5-7. | |
| Authors: | JIN Tian-ming GAO Feng TONG Qing-bin WU Ying-hong |
| Institution: | 1. Department of Animal Science, Tianjin Agricultural College, Tianjin 300384, China; College of Animal Science and Veterinary Science, Jilin University, Changchun 130062, China; 3. Jiangnan Park Jilin City, Jilin 132000, China; g. Inner Mongolia University for Nationalities, Tongliao 028000, China |
| Abstract: | We designed and synthesized a couple of primers according to pastuerella gene bank.The sequences of StrA and SulⅡwere amplified with the 4 pastuerella strains isolated clinically.Cloned gene sequences had high homology as the standard(NC_001774) strain.The result displayed that the differences of streptomycin and sulfonamide drug resistance were related to StrA and SulⅡ gene mutation.All the StrA genes were mutated in 204 and SulⅡ genes was mutated in 159,246,384,584,590,591,597,582 respectively.The mutated genes were main factors that produced streptomycin and sulfonamide resistance in Pastuerella. |
| Keywords: | Pastuerella multocida StrA gene SulⅡ gene gene mutation the mechanism of drug resistance |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |