| 超声波辅助提取大豆总木脂素及其含量分析 |
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| 引用本文： | 陈韵,石展望,黄晓敏.超声波辅助提取大豆总木脂素及其含量分析[J].大豆科学,2010,29(1). |
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| 作者姓名： | 陈韵 石展望 黄晓敏 |
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| 作者单位： | 韶关学院,生物科学系,广东,韶关,512005;广西民族大学,化学与生态工程学院,广西,南宁,530006 |
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| 基金项目： | 广东省科技计划资助项目(2005B33001004) |
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| 摘 要： | 以乙醇为浸提溶剂,超声波辅助提取不同品种大豆中的木脂素,并用紫外-可见分光光度法对其含量进行分析,同时对浸提溶剂、料液比、浸提时间及次数等因素进行比较。结果表明:以乙醇为浸提溶剂,料液比在1/8~1/12范围,浸提时间20 min,浸提2次效果比较理想;标准对照品在0~0.18 mg.mL-1浓度范围内吸光度与其总木脂素含量呈良好的线性关系,回归方程A=6.081c+0.0445,相关系数r=0.9998,总木脂素检出限8.3×10-5mg.mL-1,平均回收率为94.4%,RSD为1.2%。该方法简便、实用、可靠,可对大豆木脂素的深入研究提供参考。
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| 关 键 词： | 大豆 总木脂素 超声波辅助提取 紫外-可见分光光度法 |
Ultrasonic-assisted Extraction of Total Lignans and Its Content Analysis in Soybeans |
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| CHEN Yun,SHI Zhan-wang,HUANG Xiao-min.Ultrasonic-assisted Extraction of Total Lignans and Its Content Analysis in Soybeans[J].Soybean Science,2010,29(1). |
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| Authors: | CHEN Yun SHI Zhan-wang HUANG Xiao-min |
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| Institution: | 1.Bioscience Department of Shaoguan University;Shaoguan 512005;Guangdong;2.Chemical and Ecological Engineering College of Guangxi Ethnic University;Nanning 530006;Guangxi;China |
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| Abstract: | Total lignans in several soybean samples were detected by UV-Vis with the ultrasonic-assisted extraction in this paper.The result showed that by twice extraction it can get ideal effect with ethanol as extracting reagent in the material/reagent ratio of 1/8～1/12.The relationship between standard substance concentration within 0～0 18 mg · mL~(-1) and absorbance remained better linear.The regression equation was A=6.081c+0.0445,with the correlation coefficient r=0 9998,the detection limit was 8.3×10~(-5) mg · mL~(-1)with the average recovery of 94.4% and the RSD of 1.2%.Results suggest the method is convenient and practical to detect lignans from soybean. |
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| Keywords: | Soybean Total lignans Ultrasonic-assisted extraction UV-Vis |
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