杆状病毒J 结构域蛋白基因的克隆、表达和抗体的制备

摘要：斜纹夜蛾多粒包埋型核型多角体病毒（Spodoptera litura multicapsid nucleopolyhedrovirus，SpltMNPV）bjdp (baculovirus J domain protein) 基因是杆状病毒基因组中唯一编码J 结构域蛋白的基因。利用PCR方法扩增得到此全长基因，将其克隆到5'端有6×His编码序列的原核表达载体pQE30上并转化E. coli M15[pREP4]， 在IPTG诱导下表达了一个分子量为36 kD的融合蛋白。以纯化过的6×His-BJDP融合蛋白为抗原，免疫新西兰大白兔，得到该蛋白的抗血清。免疫印迹分析表明，该抗血清能与感染斜纹夜蛾多粒包埋型核型多角体病毒的细胞蛋白样品发生特异性反应。

Cloning and Expression of a Baculovirus J Domain Protein Gene in E. coli and Preparation of Antiserum

Abstract: Spodoptera litura multicapsid nucleopolyhedrovirus bjdp (baculovirus J domain protein ) gene is the only gene encoding a J domain protein homology in known baculovirus genome. Sequence analysis indicated that the N terminus of the gene production had a conserved J domain. With the specific primers which designed on bjdp gene reported recently, the coding region was amplified from SpltMNPV genome DNA via PCR . The PCR product was cloned into the pGEM-T Easy vector to get the recombinant plasmid (pGT-B). The bjdp gene was recombined in vitro with expression vector pQE30 and transformed into E.coli M15[pREP4]. The M15[pREP4] containing bjdp recombinant plasmid expressed a 36 kD 6×His-tag fusion protein after the induction with 1mmol/L IPTG. The fusion protein was purified with Ni-NTA resin column and used as the immunogen to raise a BJDP-specific antiserum. Western blot analysis indicated that the antiserum could react with the corresponding protein existed in SpltMNPV-infected S1-zsu-1 cells.