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Transgenic potato plants expressing oxalate oxidase have increased resistance to oomycete and bacterial pathogens |
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| Authors: | M Schneider E Droz Pia Malnoë Catherine Chatot E Bonnel J -P Métraux |
| Institution: | (1) Département de Biologie-Biologie végétale, Université de Fribourg, ch. du musée 10, CH 1700 Fribourg, Switzerland;(2) Present address: Swiss Institute of Bioinformatics, Swiss-Prot, CMU, 1 Michel-Servet, CH 1211 Geneva 4, Switzerland;(3) Station fédérale de Changins (RAC), Route de Duillier, Case postale 254, CH-1260 Nyon, Switzerland;(4) Germicopa, allée Loeiz Herrieu, F-29334 Quimper Cedex, France |
| Abstract: | Summary Potato (cv. Bintje) was transformed with a gene encoding an oxalate oxidase from wheat under the control of the CaMV35S promoter. Transgenic potato plants produced high constitutive levels of H2O2 as visualized by 4-chloro-l-naphtol staining. The resistance of these plants was tested againstPhytophthora infestans. An increased level of resistance to the disease was marked by a reduced number of lesions as well as by a decreased number of sporangia formed per lesion. In addition, oxalate oxidase overexpressing plants also exhibited improved resistance toStreptomyces reticuliscabiei, the causal agent of netted scab. Increased expression of oxalate oxidase had no effect on the interaction withErwinia carotovora. These experiments show that overexpression of oxalate oxidase represents a potentially interesting approach for protection of potato to pathogens. |
| Keywords: | Phytophthora infestans resistance Streptomyces reticuliscabiei oxalate oxidase potato |
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