| 黄海黄杆菌YS-9412-130低温碱性蛋白酶发酵过程动力学研究 |
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| 引用本文： | 孙谧,王跃军,李民,张云波,洪义国,阎晓玲. 黄海黄杆菌YS-9412-130低温碱性蛋白酶发酵过程动力学研究[J]. 海洋水产研究, 2001, 22(3): 14-23 |
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| 作者姓名： | 孙谧 王跃军 李民 张云波 洪义国 阎晓玲 |
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| 作者单位： | 1. 中国水产科学研究院黄海水产研究所
2. 中国科学院生物工程中心 |
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| 基金项目： | 国家“海洋 86 3”项目 ( 86 3-819-0 6 -0 1)资助 |
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| 摘 要： | 应用自动控制发酵设备，对黄海黄杆菌YS－9412－130碱性蛋白酶发酵动力学和工艺条件进行了研究。从基本的动力学概念和方程出发，通过分批发酵试验摸索了黄海黄杆菌YS－9412－130生长与代谢的基本规律，证明了发酵过程中低温碱性蛋白酶的形成为生长部分关联型。采用补料分批培养方法限制生长基质浓度，确定其一系列数值，从而推导出细胞生长与产物合成的动力学数学模型。
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| 关 键 词： | 黄海黄杆菌 YS－9412－130 海洋低温碱性蛋白酶 发酵动力学 补料分批培养 |
| 修稿时间： | 2000-11-10 |
Kinetic model of low-temperature alkaline protease fermentation process from Flavobacterium YS-9412-130 |
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| SUN Mi WANG Yue jun LI Min ZHANG Yun bo HONG Yi guo YAN Xiao ling. Kinetic model of low-temperature alkaline protease fermentation process from Flavobacterium YS-9412-130[J]. Marine Fisheries Research, 2001, 22(3): 14-23 |
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| Authors: | SUN Mi WANG Yue jun LI Min ZHANG Yun bo HONG Yi guo YAN Xiao ling |
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| Affiliation: | SUN Mi 1 WANG Yue jun 1 LI Min 2 ZHANG Yun bo 1 HONG Yi guo 1 YAN Xiao ling 1 |
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| Abstract: | With autocontrol fermentation equipment, the kinetic model and the technology of low temperature alkaline protease fermentation and metabolism of Flavobacterium YS 9412 130 were studied. Based on kinetic concept and equation,the basic law of the growth and metabolism of Flavobacterium YS 9412 130 was researched with batch fermentation test.The data showed that the fermentation type was one partially related to the growth. A series of parameter values were obtained by controlling the concentration of the growth limiting substrate with the fed batch culture.Thereby, the kinetic mathematical model was derived to describe the relationship between cell growth rate and product synthesis rate. |
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| Keywords: | Flavobaterium YS 9412 130 Marine low temperature alkaline protease Kinetics on fermentation process Fed batch culture |
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