| 苹果叶片再生体系建立研究 |
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| 引用本文： | 梁海永,姚伟明,杜鸿云,郑均宝,杨敏生.苹果叶片再生体系建立研究[J].河北林果研究,2005,20(3):247-250. |
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| 作者姓名： | 梁海永 姚伟明 杜鸿云 郑均宝 杨敏生 |
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| 作者单位： | 1. 河北农业大学,河北,保定,071000
2. 河北省滦县杨柳庄镇,河北,唐山,063700 |
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| 摘 要： | 以乔纳金苹果试管苗叶片为外植体诱导不定芽再生，在培养基中添加不同浓度TDZ与NAA或IAA配合，使用琼脂或Polygel作为固化剂。结果表明，较适宜的叶片再生不定芽的培养基为TDZ 2．0mg／L和NAA 1．0mg／L，或TDZ 2．0mg／L与IAA 4．0mg／L。较适宜的组培固化剂为5．0g／L的Polygel。在不同的组培固化剂中，卡那霉素均能抑制不定芽的发生数量，但琼脂和Polygel效果不同。
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| 关 键 词： | TDZ 苹果 组培固化剂 卡那霉素 不定芽 |
| 文章编号： | 1007-4961(2005)03-0247-04 |
| 修稿时间： | 2004年12月16 |
System establishment of adventitious shoot regeneration from leaves of apple (Malus domestica) in vitro |
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| LIANG Hai-yong,YAO Wei-ming,DU Hong-yun,ZHENG Jun-bao,YANG Min-sheng.System establishment of adventitious shoot regeneration from leaves of apple (Malus domestica) in vitro[J].Hebei Journal of Forestry and Orchard Research,2005,20(3):247-250. |
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| Authors: | LIANG Hai-yong YAO Wei-ming DU Hong-yun ZHENG Jun-bao YANG Min-sheng |
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| Institution: | LIANG Hai-yong~1,YAO Wei-ming~2,DU Hong-yun~1,ZHENG Jun-bao~1,YANG Min-sheng~1 |
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| Abstract: | adventitious shoots were induced from leaves of apples using a regeneration medium supplemented with four levels of thidiazuron (TDZ), and NAA, and eight levels of IAA. Solid medium was prepared using 6.0 g/L Agar or 5.0 g/L Polygel. Optimum shoot regeneration of leaf explants was obtained in a medium contain NAA 1.0 and TDZ 1.0, or IAA 4.0 and TDZ 2.0. Optimum culture gelling agent was 5.0%g/L Polygel. Adventitious shoots were reduced by the medium supplemented with varying contentrations Kanamycin. But agar and polygel was difference. |
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| Keywords: | TDZ apple gelling agent Kanamycin adventitious shoots |
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