黄瓜植株体内苯丙烯酸提取方法的研究

以黄瓜为试材,以苯丙烯酸为酚酸的代表,研究了黄瓜植株体内苯丙烯酸的提取方法。结果表明,与传统的水浸泡法相比,超声法极大地缩短了浸提时间,而且增强了浸出效果,同时,操作简单安全易行。用超声法提取黄瓜叶片和根中苯丙烯酸的最佳时间均为15 min,提取率比传统的水浸泡法分别提高了59.63%和74.54%;提取茎中苯丙烯酸的最佳时间为20 min,比传统水浸泡法提高93.59%。同时,通过对实验条件的精确控制,建立了具体超声处理黄瓜根、茎、叶组织的准确作用时间体系,为进一步测定植物体内酚酸,提供了更好的提取方法。

The extraction method of cinnamic acid in cucumber plant

The experiment was conducted to research the extraction method of cinnamic acid in cucumber plant,regarding cinnamic acid as phenolic acid.The result showed that supersonic extraction shortened the extraction tine,enhanced the extraction effect,and was easily operated by comparison with traditional water extraction by suoersonic extraction,the best time was 15 min to extract cinnamic acid in cucumber leaf and root and the extraction rate was nespectively raised 59.63% and 74.54% than water extraction: the best time is 20 min to extract cinnamic acid in cucumber stem and the extraction race was raised 93.59%.At the same tine,with strictly controlliny the experiment condition,the time system was established that the cucumber root,stem and leaf were by supersonic,so,the experiment offered a better extraction method to determine phenolic acid of the plant.