| 玉米花粉总RNA提取方法的比较和分析 |
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| 引用本文： | 黄雪梅,张守涛,杨超,王芳.玉米花粉总RNA提取方法的比较和分析[J].河南农业科学,2009(4). |
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| 作者姓名： | 黄雪梅 张守涛 杨超 王芳 |
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| 作者单位： | 郑州大学,生物工程系,河南,郑州,450001 |
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| 摘 要： | 以玉米花粉为材料,借鉴其他植物RNA的提取方法,比较了利用Trizol法、酸性酚—异硫氰酸胍法和CTAB改进法提取玉米花粉RNA的效果,利用普通琼脂糖凝胶代替甲醛变性胶电泳分析3种不同方法所提取的RNA的完整性,并用紫外分光光度计分析RNA的纯度。结果表明,CTAB改进法为其中最好的方法。
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| 关 键 词： | 玉米花粉 总RNA 提取方法 |
Comparison of Different Methods for Total RNA Extraction from Maize Pollen |
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| HUANG Xue-mei,ZHANG Shou-tao,YANG Chao,WANG Fang.Comparison of Different Methods for Total RNA Extraction from Maize Pollen[J].Journal of Henan Agricultural Sciences,2009(4). |
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| Authors: | HUANG Xue-mei ZHANG Shou-tao YANG Chao WANG Fang |
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| Institution: | Department of Bioengineering;Zhengzhou University;Zhengzhou 450001;China |
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| Abstract: | The maize pollens were used to extract total RNA using 3 different methods(Trizol,acid guanidinium isothiocyanate-phenol-chloroform and modified CTAB) and their extraction effects were compared,with reference to the RNA isolation method for other plants.The agarose gel electrophoresis,instead of formaldehyde denaturalization electrophoresis,was used to analyze the integrality of RNA extracted by 3 methods,and the UV-spectrophotometer was used to analyze the purity of RNA.The result showed that the modified ... |
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| Keywords: | Maize pollen Total RNA Extracting method |
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