禽流感病毒M2基因在原核系统中表达及其抗原性分析

M2蛋白是禽流感病毒的跨膜蛋白，该结构蛋白具有潜在的抗原性，是禽流感病毒的3种表面抗原之一，在机体内不仅能刺激产生抗体，而且能引起CTL反应。由于其氨基酸序列高度保守，因此以M2蛋白作为抗原，可对不同亚型的禽流感毒产生一定的保护性免疫。

Expression of Avian Influenza Virus M2 Gene in E.coli and Analysis of Its Antigenicity

Avian influenza virus (AIV) is a highly variable pathogen. The M2 gene of AIV is a transmembrane protein. It has highly conservative antigenic epitopes and is a potential antigen for cross-protection. In this study, the recombinants containing the full-length M2 and the transmembrane segment deleted M2 were constructed respectively. When measuring the expression of the two constructs, we found that the full-length M2 failed to express in the prokaryotic expression system, whereas transmembrane segment deleted M2 was highly expressed as fusion protein in a soluble form. The fusion protein reacted with SPF chicken serum specific for AIV A/goose / Guangdong /1996(H5N1). The protein was purified by GST purification system and the purified protein was used to prepare anti-M2 serum in mice. Immunofluorescence test demonstrated the binding of the antiserum with AIV (H5N1) infected MDCK cells. The results suggest that the recombinant transmembrane segment deleted M2 could provide good antigenicity.