DTA-6对大豆花荚脱落纤维素酶和GmAC基因表达的调控

叶面喷施生长调节剂(PGRs)可以调控大豆花荚脱落。2012-2013年在大庆林甸县黑龙江八一农垦大学试验基地采用大田小区试验，R1期对大豆绥农28(SN28)、垦丰16 (KF16)和合丰50 (HF50)分别叶面喷施DTA-6调节剂，对调控花荚离区脱落纤维素酶(GmAC)基因相对表达量、荚脱落纤维素酶(AC)活性、花荚脱落率和产量进行了研究。结果表明：DTA-6抑制大豆花荚离区GmAC基因相对表达量，最大可达51% (±CK%)；大豆荚的AC活性能够在喷药后阶段性地被降低，在不同品种上增加和降低存在差异；能够显著降低花荚脱落率(P<0.05)；能够显著增加产量(P<0.05)。植物生长调节剂DTA-6是通过降低花荚离区GmAC基因相对表达量，调节AC活性，从而减少植株的花荚脱落率，以实现对产量的调控。

Regulation of DTA-6 by Abscission Cellulase and GmAC Gene Expression in Flowers and Pods of Soybean

It is effective to regulate abscission of soybean flowers and pods by spraying plant growth regulators (PGRs). This study was carried out to determine the effect of DTA-6 on abscission cellulose (AC) activity, abscission cellulose (GmAC) gene expressive, abscission of flowers and pods and yield in soybean. DTA-6 was foliage sprayed at R1 stage on three varieties of Suinong 28 (SN28), Kenfeng 16 (KF16), and Hefeng 50 (HF50) in 2012 and 2013. Our results demonstrated that DTA-6 treatment inhibited GmAC gene expression in abscission zone of flower and pod, with the maximum reduction of 51% among three varieties compared with control. The abscission cellulase activity was periodically decreased by DTA-6, with different decrements among soybean cultivars. DTA-6 significantly decreased (P<0.05) abscission rate of soybean flowers and pods. And significantly increased yield (P<0.05). The relative expression of abscission cellulase gene (GmAC) in abscission zone of flowers-pods was decreased and AC activity was regulated by DTA-6, resulting in reduced abscission rate of soybean flowers and pods and promoted yield.