Proteomic analysis of a compatible interaction between Pisum sativum (pea) and the downy mildew pathogen Peronospora viciae |
| |
Authors: | R C Amey T Schleicher J Slinn M Lewis H Macdonald S J Neill P T N Spencer-Phillips |
| |
Institution: | (1) Centre for Research in Plant Science, Faculty of Health and Life Sciences, University of the West of England, Coldharbour Lane, Bristol, BS16 1QY, UK |
| |
Abstract: | A proteomic approach was used to identify host proteins altering in abundance during Peronospora viciae infection of a susceptible cultivar of pea (Pisum sativum cv. Livioletta). Proteins were extracted from fully developed pea leaflets at 4 days post-inoculation, before visible symptoms
were apparent. Cytoplasmic proteins and membrane- and nucleic acid-associated proteins from infected and control leaves were
examined using two-dimensional difference gel electrophoresis. The majority of proteins had a similar abundance in control
and infected leaves; however, several proteins were altered in abundance and twelve were found to have increased significantly
in the latter. These proteins were selected for either matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry
or electro-spray ionisation quadrupole time-of-flight tandem mass spectrometry analysis following trypsin digestion, with
sequence identity being assigned to eight of the proteins. These included the ABR17 stress-response protein, the pathogen-induced
PI176 protein, three photosynthetic proteins, a glycine-rich RNA binding protein and two glyceraldehyde 3-phosphate dehydrogenases
(cytosolic and chloroplastic) which can be induced by a range of abiotic and biotic stresses in many plant species. The possible
roles of these proteins in the response of the pea plant during P. viciae infection are discussed. This study represents the first proteomic analysis of downy mildew infection of pea leaves, and
provides the basis for further work to elucidate molecular mechanisms of compatibility in P. viciae infections. |
| |
Keywords: | Electrophoresis DIGE MALDI-TOF Mass spectrometry Oomycete Protein |
本文献已被 SpringerLink 等数据库收录! |
|