芥菜型油菜与白菜种间杂种的获得与鉴定

以芸薹属植物芥菜型油菜和白菜为研究对象，对其进行常规正反杂交后子房的离体培养，通过离体子房培养获得的杂种种子经MS培养基诱导成苗，获得了芥菜型油菜与白菜的杂种F1代植株，表现出杂种优势；通过细胞学的方法，鉴定了杂种F1幼蕾体的细胞染色体数为2n=28，为母本芥菜型油菜（2n=36）和父本白菜（2n=20）的单倍体染色体数之和；芥菜型油菜与白菜常规杂交后，经离体子房培养获得了“胚性芽”，确立了植物胚挽救的另一种形式。

Access and Identification of Intergeneric Hybrid Between Brassica junce×B.pekinesis

The research studies the plant of rape genus, Brassica junce and B. pekinesis. By removed ovary culture after positive and negative hybridization, produced interspecific hybridization, and have improved the hybrid seed through removed ovary culture and induced to shoot by culture medium MS, and produced hybrid F1, the plant of Brassica junce×B.pekinesis, the advantage characteristic of hybrid plant is similar to female nature；By cytology authenticating, chromosome number of F1 young flower bud cell (2n=28), it is the sum of monoploid chromosome number of female parent Brassica junce(2n=36) and male parent B.pekinesis(2n=20); The hybridization to Brassica junce×B.pekinesis, and embryonic bud come from removed ovary culture, and it found another form of plant embryo saving.