利用花托与花序轴组培快繁青花菜雄性不育株的研究

以青花菜的花托和花序轴作外植体进行组织培养,可成功诱导出苗,并以此来保存突变不育株。试验表明:不同的青花菜品种在同一种培养基上诱导率差别很大(2.3%~83.3%),05A-743-2在MS+BA 0.2mg/L+KT0.2mg/L+NAA0.01mg/L培养基上花托诱导率最高可达83.3%,花序轴诱导率达72.2%;用MS+BA 0.5mg/L+NAA 0.002mg/L继代培养,苗正常、健壮,增殖系数可达5.98;用1/2MS+IBA 0.02mg/L进行生根培养,生根率达91.7%,平均根数13.0条;利用全光照自动喷雾过渡培养组培苗,成活率达100%,组培苗可瓶外生根,效果比瓶内生根好。

Studies on Tissue Culture of Receptacles and Rachides, and Consequent Rapid Multiplication of Male Sterile Lines in Brassica oleracea L. var. italica Phenck

Tissue culture of Brassica oleracea L.var.italica Phenck,which was done with its receptacles and rachides as explants,succeeded in seedling inducement.Datum indicated that different varieties of the vegetable had significant difference in inducing rate(2.3%～83.3%) on the same culture medium,and 05A-743-2 had the uppermost receptacle inducing rate of 83.3% and the rachis inducing rate of 72.2%,both on MS+BA 0.2mg/L+KT 0.2mg/L+NAA 0.01mg/L culture medium.Seedlings showed favorable growth after being cultivated consequently on MS+BA0.5mg/L+NAA0.002mg/L with a proliferation coefficient of 5.98.The rhizogenic rate was 91.7% with 13 pieces of roots in average on 1/2MS+IBA 0.02mg/L.Seedlings' survival rate reached 100% by full-light,automatic-spraying and transitionary culture methods.Moreover,the effect of rhizogenesis outside bottles was better than that inside bottles.