粗山羊草全基因组Aux/IAA基因家族的分离、染色体定位及序列分析

生长素是植物生长发育过程中的关键激素之一，Aux/IAA家族基因是重要的生长素原初响应基因。通过生物信息学方法从粗山羊草(Aegilops tauschii)全基因组中分离出28个Aux/IAA基因，其中20个粗山羊草Aux/IAA蛋白具有4个保守结构域；28个Aux/IAA基因分布于全部7对染色体上，5个基因分别具有位于同一位点的已知标记。粗山羊草IAA3、IAA11和IAA26的表达具有组织特异性，分别在雌蕊、种子和根中特异表达。系统发育显示，11对粗山羊草-乌拉尔图小麦Aux/IAA蛋白、5对粗山羊草-大麦Aux/IAA蛋白直系同源。共线性分析表明，粗山羊草Aux/IAA基因与短柄草、水稻中同源基因具有很好的共线性。本研究分离的相关基因不仅可用于小麦的遗传改良，也为深入研究小麦Aux/IAA基因提供了信息。

Whole-Genome Sequence Isolation,Chromosome Location and Characterization of Primary Auxin-Responsive Aux/IAA Gene Family in Aegilops tauschii

Auxin, as one of the most important hormones, plays a key role in many processes of plant development. The Aux/IAA family contains important early auxin response genes. A genome-wide research of Aux/IAA genes in Aegilops tauschii was carried out using bioinformatic method. In this study, 28 Aux/IAA genes were identified in Ae. tauschii, which were distributed on seven chromosomes of Ae. tauschii genome. Twenty Aux/IAA genes share four conserved amino acid sequence motifs and five were mapped on the same locus with known markers. AetIAA3, AetIAA11, and AetIAA26 were specifically expressed in pistil, seed and root of Ae. tauschii, respectively. Eleven pairs of Ae. tauschii–Triticum urartu and five pairs of Ae. tauschii–Hordeum vulgare Aux/IAA proteins were orthologous in the phylogenetic tree. Collinearity analysis indicated Aux/IAA genes showed a higher synteny between Ae. tauschii and two other species (Brachypodium distachyon and Oryza stativa). The Aux/IAA genes isolated can not only be applied in genetic improvement of common wheat but also provide basic information in further research of Aux/IAA genes in wheat.