表皮生长因子对水牛卵母细胞体外培养核质成熟的影响

为了探讨表皮生长因子（EGF）对水牛卵泡卵母细胞体外培养核质成熟的影响，在以TCM199为基础的成熟液中加入不同浓度的EGF（0、10、25、50、100 ng/ml），体外成熟培养24~26 h，观察第一极体（PB1）的排放；随后进行孤雌激活检测其分裂率、囊胚发育率、囊胚孵化率，并用Hoechst33342染色后计算囊胚的细胞数。结果发现添加EGF各组的卵母细胞第一极体排放率显著提高（P<0.05）；成熟液中添加25 ng/ml EGF时，卵裂率及囊胚发育率（分别为80.0%、44.8%）明显高于对照组（分别为69.3%、31.9%，P<0.05），但对囊胚的细胞数影响不大。EGF不仅促进水牛卵母细胞体外培养的核成熟，而且有利于卵母细胞的胞质成熟，其中EGF的最佳浓度为25 ng/ml。

Influence of Epidermal Growth Factor on the Nuclear and Cytoplasm Maturation of Buffalo Oocytes in Vitro Culture

To examine the effects of epidermal growth factor (EGF) on the nuclear and cytoplasm maturation of buffalo oocytes in vitro culture. After the buffalo oocytes were matured in vitro for 24~26 h in TCM199 control medium supplied with EGF（0, 10, 25, 50, 100 ng/ml）, the first polar body (PB1) formation was assessed, then the oocytes were activated by 6 dimethylaminopurine combined with ionomycin, examining the cleavage rate、blastocyst development rate and the number of blastocyst by staining with Hoechst33342. Adding EGF to the culture medium improved the nuclear maturation rate of buffalo oocytes significantly (P<0.05). The cleavage rate, blastocyst development rate and blastocyst hatching rate adding 25 ng/ml EGF to medium group were significantly higher than those in control group; but there were no significant difference in the number of cells of blastocyst. EGF can improve the effect of maturation of nuclear and cytoplasm of buffalo oocytes in vitro culture, and the best concentration of EGF was 25 ng/ml.