传染性法氏囊病病毒的PCR引物设计与Internet检索分析

研究了传染性法氏囊病病毒（ＩＢＤＶ）Ａ片断的ｃＤＮＡ的结构，并使用ＰｒｉｍｅｒＤｅｓｉｇｎ引物设计软件，在ＶＰ５和ＶＰ２的重叠基因区设计了一对引物，各种ＩＢＤＶ毒株在此部位有非常高的同源性，在对引物进行Ｉｎｔｅｒｎｅｔ检索中得到了证实。经过对４种ＩＢＤＶ的疫苗标准毒株，１种野毒株，１例病鸡标本试检测，验证引物设计符合要求，具有良好的适应性和较高的灵敏度。

Design of Infectious Bursal Disease Virus PCR Primer and Interne t Retrieval

Based on the study of segment A of the infectious bursal disease virus(IBDV) cDNA, a pair of primers was designed with the PrimerDesign software. This pair of primers is in the overlapping area of VP 5 and VP 2 where the sequence is highly homologous, which was verified when searching them in the Internet. Four standard virus strains, one wild strain and one IBDV strain from the cloacal bursa of diseased chicken had been amplified successfully with the primers. Two other viruses, which could result in other disease of chicken and one healthy chicken's cloacal bursa had given negative results. This proved that the primers accord with the demand of the amplification of IBDV,and have good adjustability and high sensitivity.