Apoptotic intrinsic pathway proteins predict survival in canine cutaneous mast cell tumours |
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| Authors: | C N Barra B M Macedo K G Cadrobbi L H Pulz G C Huete S R Kleeb J G Xavier J L Catão‐Dias A T Nishiya H Fukumasu R F Strefezzi |
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| Institution: | 1. Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de S?o Paulo, S?o Paulo, Brazil;2. Laboratório de Oncologia Comparada e Translacional, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de S?o Paulo, Pirassununga, Brazil;3. Universidade Metodista de S?o Paulo, S?o Bernardo do Campo, Brazil;4. Universidade Paulista, S?o Paulo, Brazil;5. Universidade Anhembi Morumbi, S?o Paulo, Brazil |
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| Abstract: | Mast cell tumours (MCTs) are the most frequent canine round cell neoplasms and show variable biological behaviours with high metastatic and recurrence rates. The disease is treated surgically and wide margins are recommended. Adjuvant chemotherapy and radiotherapy used in this disease cause DNA damage in neoplastic cells, which is aimed to induce apoptotic cell death. Resisting cell death is a hallmark of cancer, which contributes to the development and progression of tumours. The aim of this study was to investigate the expression of the proteins involved in the apoptotic intrinsic pathway and to evaluate their potential use as prognostic markers for canine cutaneous MCTs. Immunohistochemistry for BAX, BCL2, APAF1, Caspase‐9, and Caspase‐3 was performed in 50 canine cases of MCTs. High BAX expression was associated with higher mortality rate and shorter survival. BCL2 and APAF1 expressions offered additional prognostic information to the histopathological grading systems. The present results indicate that variations in the expression of apoptotic proteins are related to malignancy of cutaneous MCTs in dogs. |
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| Keywords: | apoptosis
BAX
BCL2
dog immunohistochemistry prognosis |
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