3种染色体组加倍方法诱导黄河鲇雌核发育的比较

用经紫外线照射后遗传失活的黄河鲇精子、刺激性成熟的黄河鲇卵子, 进行雌核发育, 采用冷休克、热休克和秋水仙素浸泡等3种染色体组加倍方法诱导黄河鲇卵进行二倍体雌核发育。实验结果表明: 在人工诱导黄河鲇二倍体雌核发育过程中, 以冷休克处理组的效果最好, 胚胎孵化率达到8. 1%; 其次为秋水仙素处理组, 胚胎孵化率达到6. 3%; 热休克处理组诱导效果较差, 胚胎孵化率仅为4. 0%。

The Contrastive Study on Induction of Gynogenesis by Three Different Methods of Chromosome Doubling in Silurus asotus

The Silurus asotus eggs were activated by UV-irradiated Silurus asotus sperm, chromosome doubling was made on the induction of gynogenesis in Silurus asotus by cold-shocking ,hot-shocking and colchicines. The study showed that optimum method for chromosome doubling in induction of gynogenesis in Silurus asotus was cold-shocking , the hatch rate may reach 8.1% , the second fine method was the induced by colchicines, and it's hatch rate was 6.3%, the hot-shocking method for chromosome doubling in induction of gynogenesis in Silurus asotus was worst, it's hatch rate only reached 4.0%.