辣椒疫霉菌菌种保存及致病性研究

在辣椒疫病抗性材料鉴定和疫霉菌生理小种测定等工作中， 常因菌种在室内连续多代转接培养导致菌株的致病力下降， 影响测定结果的准确性和一致性。针对辣椒疫霉菌的长时间保存这一问题， 通过对 4个辣椒疫霉菌株的长时间无菌水保存和常规固体培养基斜面连续转接保存方法进行试验对比，定期对菌株培养性状和致病力进行观察和测定。试验结果表明： 在 16℃条件下， 无菌水中保存的辣椒疫霉菌株在 24个月后仍有活力， 菌株培养性状没有发生变化， 致病性也没有明显衰退。该菌种保存方法制作简单、 经济、 可靠， 适用于辣椒疫霉菌种的长期保存。

Research on Preservation of the Phytophthora capsici and Its Pathogenicity

The pathogen of pepper phytophthora blight, phytophthora capsici, usually degenerates as it is successively transplanted and cultivated indoors during identification of resistance and physiological race, which affects the accuracy of identification. In order to maintain the pathogenicity, four strains were cultivated in aseptic water and solid medium, the colony morphology and pathogenicity were measured every three months. The results indicated that the preservation effect of aseptic water at 16℃ was better than that of solid medium. The four strains all had viability when they were preserved for 24 months, the colony morphology and pathogenicity also did not change. This method of preserving pathogens was simple, economical and reliable, so it might be used for strains long-term preservation.