| 赤桉GAPDH家族基因的克隆及其序列分析 |
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| 引用本文： | 陈鸿鹏,朱凤云,吴志华,谢耀坚. 赤桉GAPDH家族基因的克隆及其序列分析[J]. 林业科学研究, 2014, 27(1): 120-127 |
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| 作者姓名： | 陈鸿鹏 朱凤云 吴志华 谢耀坚 |
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| 作者单位： | 1.国家林业局桉树研究开发中心, 广东 湛江 524022;2.黄淮学院生物工程系, 河南 驻马店 463000 |
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| 基金项目： | 湛江市科技计划项目(2013A03016);林业重大公益性行业专项(201104003);广东省自然科学基金项目(91456245364000003) |
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| 摘 要： | 正甘油醛-3-磷酸脱氢酶(GAPDH)是高等植物糖酵解和糖异生反应中的关键酶,是维持生命活动能量形成的最基本酶之一,它氧化甘油醛-3-磷酸生成1,3-二磷酸甘油酸,由此为糖酵解过程提供产生ATP的底物[1-4]。此外,由于该酶基因为管家基因,几乎在所有组织中都高水平表达,在同种细胞或组织中的蛋白质表达量较为恒定,故被广泛用作实时荧光定量PCR的内参基因[5-9]。近年来,随着研究
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| 关 键 词： | 赤桉 GAPDH 家族基因 克隆 序列分析 |
| 收稿时间： | 2013-07-02 |
Cloning and Sequence Analysis of GAPDH Family Genes from Eucalyptus camaldulensis |
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| CHEN Hong-peng,ZHU Feng-yun,WU Zhi-hua,XIE Yao-jian. Cloning and Sequence Analysis of GAPDH Family Genes from Eucalyptus camaldulensis[J]. Forest Research, 2014, 27(1): 120-127 |
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| Authors: | CHEN Hong-peng ZHU Feng-yun WU Zhi-hua XIE Yao-jian |
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| Affiliation: | 1. China Eucalypt Research Centre, Zhanjiang 524022, Guangdong, China;2. Department of Agronomy-Forestry Science, Huanghuai University, Zhumadian 463000, He'nan, China |
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| Abstract: | GAPDH is a typical structural protein in eukaryote organism, which plays an important role involved in the biosynthesis of cell composition and the expression of genetic information. In order to reveal the biological function of GAPDH in the growth and development of Eucalyptus, 3 genes were obtained and named as EC-GAP1, EC-GAP2 and EC-GAP3 by amplification with primers for GAPDH genes conserved regions and RACE method from the young leaf of Eucalyptus camaldulensis. The bioinformatics analysis suggested that the proteins encoded by corresponding genes owned the typical conserved domains of GAPDH and showed high homology with those of other plant species. |
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| Keywords: | Eucalyptus camaldulensis< em> GAPDH< em> family genes cloning sequence analysis |
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