| 甘薯NPR1基因半定量RT-PCR检测方法的建立 |
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| 引用本文： | 陈观水,潘大仁,周以飞,林生,高丽华,杨志伟.甘薯NPR1基因半定量RT-PCR检测方法的建立[J].福建农林大学学报(自然科学版),2007,36(1):56-59. |
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| 作者姓名： | 陈观水 潘大仁 周以飞 林生 高丽华 杨志伟 |
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| 作者单位： | 1. 福建农林大学生命科学学院
2. 福建农林大学作物科学学院,福建,福州,350002 |
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| 基金项目： | 福建省自然科学基金
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福建省教育厅科研项目 |
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| 摘 要： | 为进一步研究甘薯病原微生物的侵染对甘薯病程相关非表达子1基因(NPR1)表达的影响,以甘薯肌动蛋白(β-ac-tin)基因为内参照基因,提取甘薯叶片总RNA,反转录为cDNA,同批异管对该2个基因进行PCR扩增.通过对循环数的优化和对体系重复性、准确性的分析,建立了一个稳定、方便的半定量RT-PCR体系.
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| 关 键 词： | 甘薯 病程相关非表达子1(NPR1) 基因表达 半定量RT-PCR |
| 文章编号： | 1671-5470(2007)01-0056-04 |
| 修稿时间： | 2006-11-25 |
Establishment of the semi-quantitative RT-PCR system of nonexpressor of pathogenesis-related genes 1 (NPR1) gene in sweet potato (Ipomoea batatas Lam.) |
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| CHEN Guan-shui,PAN Da-ren,ZHOU Yi-fei,LIN Sheng,GAO Li-hua,YANG Zhi-wei.Establishment of the semi-quantitative RT-PCR system of nonexpressor of pathogenesis-related genes 1 (NPR1) gene in sweet potato (Ipomoea batatas Lam.)[J].Journal of Fujian Agricultural and Forestry University,2007,36(1):56-59. |
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| Authors: | CHEN Guan-shui PAN Da-ren ZHOU Yi-fei LIN Sheng GAO Li-hua YANG Zhi-wei |
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| Institution: | 1. College of Life Science; 2. College of Crop Science, Fujian Agriculture and ForesuT University, Fuzhou, Fujian 350002, China |
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| Abstract: | With β-actin gene as the inner control,the total RNA of sweet potato young leaves was extracted and reversely transcribed to form cDNA to further study the expression of nonexpressor of pathogenesis-related genes 1(NPR1) gene in sweet potato suffering from pathogen stress.The fragments of the two genes were PCR amplified with the same cycle numbers during the exponential phase of NPR1 and β-actin in different tubes.Cycle number of PCR system was optimized and analyzed in their repeatability and precision,and then a stable and convenient semi-quantitative RT-PCR system was established.This system can be used to analyze the relationship between expressions of NPR1 gene in different sweet potato and pathogen,then to ascertain the expression pattern of NPR1 gene and function in sweet potato,to provide reference for the breeding of sweet potato resistant to disease. |
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| Keywords: | sweet potato nonexpressor of pathogenesis-related genes 1(NPR1) gene expression semi-quantitative RT-PCR |
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