| 亚麻外源DNA导入后代的过氧化物酶同工酶分析 |
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| 引用本文： | 王玉富,刘燕,乔广军,路颖,王殿奎,张福修,王彦荣,关东明,代贵金.亚麻外源DNA导入后代的过氧化物酶同工酶分析[J].中国麻业,1996(3). |
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| 作者姓名： | 王玉富 刘燕 乔广军 路颖 王殿奎 张福修 王彦荣 关东明 代贵金 |
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| 作者单位： | 黑龙江省农业科学院经济作物研究所，沈阳农业大学农学系，辽宁省丹东市农科所 |
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| 摘 要： | 本文讨论了亚麻过氧化物酶同工酶酶谱分析的适宜方法，在此基础上对利用花粉管通道技术进行的亚麻外源ＤＮＡ导入后代进行了过氧化物酶同工酶酶谱分析。结果表明：ＤＮＡ导入后代与其受体的酶谱差异明显，主要表现为酶谱带数的不同和谱带染色深浅的差异。由此表明外源ＤＮＡ片段（或基因）已整合到受体基因组中，并得到表达。
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| 关 键 词： | 亚麻（LinumusitatissimumL．） 外源DNA 同工酶 |
Analysis of Peroxidase Isozyme of the Progenies Derived by Introducing Exogenous DNA Into Flax(Linum usitatissimus L.) |
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| Wang Yufu, Liu Yan, Qiao Guangjun, Lu Ying, Wang Diankui, Zhang Fuxiu.Analysis of Peroxidase Isozyme of the Progenies Derived by Introducing Exogenous DNA Into Flax(Linum usitatissimus L.)[J].Plant Fibers and Products,1996(3). |
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| Authors: | Wang Yufu Liu Yan Qiao Guangjun Lu Ying Wang Diankui Zhang Fuxiu |
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| Institution: | Wang Yufu; Liu Yan; Qiao Guangjun; Lu Ying; Wang Diankui; Zhang Fuxiu(Instiute of Industrial Crops. Acadmy of Agricultural Science of Heilongjiang Province. Hulan 150518)Wang Yanrong; Guan Dongming; Dai Guijin(Shen yang Agricultural University) (Agricutur |
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| Abstract: | This paper discusses the proper methods to analyze peroxidase isozyme patterns of flax.and on the base,peroxidase isozyme patterns of the progenies derived by introducing exogenous DNA into flax through formed passage of the Pollen tube are analyzed. The results indicate that the isozyme Patterns of the transfered progenies are different from the recipients,which is shown by the number and the stainning degree of isozyme bands. This indicates that exogenous DNA segments have inserted into genomes of the recipients plants and have expressed. |
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| Keywords: | Flax Exogenous DNA Peroxidase isozyme |
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