芸薹属A基因组DNA封阻下的C染色体组核型分析

为了探索一种高效可靠的芸薹属植物核型分析方法，本文以甘蓝和白菜为实验材料，分别提取甘蓝基因组DNA用地高辛标记为探针，提取白菜基因组DNA作为封阻剂，对甘蓝有丝分裂中期染色体进行原位杂交，每对同源染色体上显示出了特定的原位杂交信号，将甘蓝的9对染色体进行了精细的分型。为区分像甘蓝这样的小型染色体提高了一条可行且精确的方法，并为研究甘蓝自交不亲和性信号传导相关基因在染色体上的定位奠定重要基础。

Karyotyping of Brassica C genome based on Brassica A genomic DNA as block reagent

To explore an effective and reliable karyptyping method in Brassica crop plants, genomic DNA was isolated from Brassica oleracea genome, labeled as probe with DIG-High Prime Mix kit; genomic DNA was isolated from Brassica rapa genome as block reagent. In situ hybridization to mitotic spreads, specific fluorescent signals were showed on each pair of homologous chromosome. Nine pairs of Brassica oleracea genome were exactly identified. It provided a feasible and accurate method to discriminate species with small chromosomes. And it set up an important found to research localization of self-incompatible genes.