| 不同激素配比对番茄下胚轴诱导与分化的影响 |
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| 引用本文： | 宗宪春,许向阳,王傲雪,张贺,李景富.不同激素配比对番茄下胚轴诱导与分化的影响[J].安徽农业科学,2010,38(35):19927-19928. |
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| 作者姓名： | 宗宪春 许向阳 王傲雪 张贺 李景富 |
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| 作者单位： | 东北农业大学园艺学院,黑龙江哈尔滨,150030;牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157012;东北农业大学园艺学院,黑龙江哈尔滨,150030 |
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| 摘 要： | 目的]研究不同激素配比对番茄下胚轴诱导与分化的影响。方法]以番茄栽培品种03HN31为试验材料,通过对番茄下胚轴的离体培养,研究不同激素配比对番茄下胚轴愈伤组织诱导、不定芽分化和根形成的影响。结果]愈伤组织诱导和芽分化的最适培养基为MS＋6-BA 2.0 mg/L＋IAA 0.2 mg/L,有利于根形成的最适培养基为MS＋IAA 0.1 mg/L。结论]该研究为番茄转基因研究、亲本的保存以及快速繁殖等工作奠定了基础。
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| 关 键 词： | 番茄 下胚轴 激素配比 诱导与分化 |
Effect of the Plant Hormone Ratio on Induction and Differentiation of Tomato's Hypocotyl |
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| Institution: | ZONG Xian-chun et al(College of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030) |
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| Abstract: | Objective] Effect of plant hormone ratio on inducion and differentiation of tomato′s hypocotyls was studied.Method] Hypocotyls of tomato were used for explant to study effect of plant hormone ratio on callus,bud induction and root formation.Result] The experiment results showed that MS+6-BA 2.0 mg/L+ IAA 0.2 mg/L was suited for callus induction and bud differentiation;MS+IAA 0.10 mg/L was suited for root formation.Conclusion]It provided basic data for the establishment of rapid propogation,indigenous resources conservation and genetic transformation of tomato. |
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| Keywords: | Tomato Hypocotyl Hormone ratio Znduction and differentiation |
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