仔猪大肠杆菌病复合多价卵黄抗体的研制与应用

将“K8 8”、“K99”、“987P”和“F41”大肠杆菌接种于产蛋母鸡获得多价高免卵黄抗体 ,与有效中药提取物和保护剂按一定比例混合 ,瞬间喷雾干燥制备的复合多价卵黄抗体干粉其抗体效价维持不变。新生仔猪攻毒及治疗试验结果表明 ,大肠杆菌性腹泻发病仔猪口服复合多价卵黄抗体干粉制剂的治愈率明显高于硫酸庆大霉素治疗的治愈率 ;临床预防试验结果表明 ,新生仔猪口服复合多价卵黄抗体干粉制剂对其大肠杆菌性腹泻的预防效果显著高于疫苗 ,表明该制剂是安全有效的抗新生仔猪大肠杆菌性腹泻的新型生物制剂

Development and application of combined multivalent egg-yolk antibodies in passive protection of piglet diarrhea caused by enterotoxicgenic E.coli

A mixture of cultures of K88-,K99-,987P- and F_41- piliate enterotoxigenic E. coliwas used to immunize hens to produce eggs containing antibodies against enterotoxigenic E.coli,from which the antibodies were extracted and purified.The purified antibodies were mixed with the Chinese traditional medicine and protectives proportionally to make up the combined multivalent chicken egg-yolk antibodies,and then produce the powder by spray drying process.In the treatment trial of infected piglets,the cure rate using the combined multivalent chicken egg-yolk antibody dry powder is higher than that of Gentamycin.In the prevention trial,the oral administration of the combined multivalent chicken egg-yolk antibodies has an higher prevention effect significantly than that of vaccine against enterotoxigenic Escherichia coli.It is concluded that the combined multivalent chicken egg-yolk antibody powder is a new,safe and effective preparation in prevention and treatment of piglet diarrhea caused by enterotoxigenic E.coli.