| 火龙果镰刀菌果腐病病原菌鉴定及生物学特性研究 |
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| 引用本文： | 朱迎迎,高兆银,李敏,陈亮,胡美姣. 火龙果镰刀菌果腐病病原菌鉴定及生物学特性研究[J]. 热带作物学报, 2016, 37(1): 164-171. DOI: 10.3969/j.issn.1000-2561.2016.01.027 |
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| 作者姓名： | 朱迎迎 高兆银 李敏 陈亮 胡美姣 |
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| 作者单位： | 1. 海南大学环境与植物保护学院,海南海口 570228;中国热带农业科学院环境与植物保护研究所 农业部热带作物有害生物综合治理重点实验室 海南省热带农业有害生物监测与控制重点实验室 海南海口 571101;2. 中国热带农业科学院环境与植物保护研究所 农业部热带作物有害生物综合治理重点实验室 海南省热带农业有害生物监测与控制重点实验室 海南海口 571101 |
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| 基金项目： | 公益性行业（农业）科研专项（No. 201303077-1）;中央级公益性科研院所基本科研业务费专项（No. 2013hzs1J012、1630062015016）。 |
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| 摘 要： | 采用形态学特征观察、rDNA-ITS序列、EF-1α序列比对和系统发育树分析的方法对引起火龙果镰刀菌果腐病病原菌进行鉴定,并初步研究其生物学特性。结果表明:引起火龙果镰刀菌果腐病是单隔镰刀菌(Fusarium dimerum Penzig in Saccardo),这是该菌引起火龙果果腐病在国内的首次报道。该菌菌丝生长的适宜温度25~35℃,最适温度30℃,适宜产孢温度25~35℃,最适产孢温度30~35℃,致死温度75℃(10 min);适宜pH5~9,最适pH7,最适产孢pH4;连续光照、D-果糖为碳源、牛肉膏和蛋白胨为氮源时最有利于该菌菌丝生长;完全黑暗、D-半乳糖为碳源、尿素为氮源时最有利于该菌产孢。综合分析认为,该菌耐高温,光照充足、弱酸和富含有机营养的环境有利于该菌的生长和繁殖。
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| 关 键 词： | 火龙果 果腐病 单隔镰刀菌 鉴定 生物学特性 |
Identification and Biological Characteristics of Dragon Fruit (Hylocereus undatus Britt)Fusarium Rot Pathogen |
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| ZHUYingying,GAO Zhaoyin,LI Min,CHEN Liang and HU Meijiao. Identification and Biological Characteristics of Dragon Fruit (Hylocereus undatus Britt)Fusarium Rot Pathogen[J]. Chinese Journal of Tropical Crops, 2016, 37(1): 164-171. DOI: 10.3969/j.issn.1000-2561.2016.01.027 |
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| Authors: | ZHUYingying GAO Zhaoyin LI Min CHEN Liang HU Meijiao |
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| Affiliation: | 1 College of Environment and Plant Protection, Hainan University,2 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture/ Haina;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture/ Hainan Key Laboratory for Monitoring and Control of Tropical Agricultura;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture/ Hainan Key Laboratory for Monitoring and Control of Tropical Agricultura;1 College of Environment and Plant Protection, Hainan University,2 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture/ Haina;College of Environment and Plant Protection, Hainan University |
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| Abstract: | |
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| Keywords: | Dragon fruit Fruit Rot Fusarium dimerum Identification Biological characteristic |
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