魔芋组织培养中不同生长阶段同工酶变化的研究

利用聚丙稀酰胺电泳分离了楚魔花1号、魔白10号和谢君魔芋和魔野14号4个种组织培养中的3个时期(苗期、生根期、结薯期)的酯酶和过氧化物酶,分析比较了2种同工酶在4个魔芋种的3个时期酶谱数量、酶活性的变化,得出了3个时期酶谱和酶活性变化的规律。结果表明:酯酶酶带数以生根期最多,苗期和结薯期的变化没有规律;酶活性在楚魔花1号、魔白10号和谢君魔芋3个魔芋种的生根期和结薯期的变化不是很大,都强于苗期的酶活性,魔野14号的酶活性则是苗期和生根期的相似,且稍大于结薯期。过氧化物酶酶带数在4个种中没有变化,只是不同生育时期酶谱迁移率略有不同;酶的活性除谢君魔芋外都呈现,结薯期最强,生根期次之,苗期最弱。谢君魔芋的酶活性变化则是苗期最强,生根期次之,而结薯期最弱。结果表明2种同工酶在魔芋的不同阶段都有选择性的表达,在一定的程度上参与了魔芋的形态建成。

Change of Isoenzyme at Different Growth and Development Stages of Tissue Culture in Amorphaphollus

The zymograms and enzymes activity of esterase isoenzymes and peroxides isoenzymes of Amorphaphollus xiei P.Y.Liu,Amorphaphollu konjac K.Coch,Amorphaphollus albus P.Y.Liu,MoYe14 Amorphaphollus sp.at 3 growth and development stages in tissue culture were studied by electrophoresis.The results were as follows: the zymograms of esterase enzymes were the most at rooting period,the change law of zymograms at seedling stage and tuber formation period were not observed;enzyme activity of esterase enzymes was the strongest at tuber formation period,the next was at rooting period,and that at seedling stage was the weakest in A.konjac K.Coch,A.xiei P.Y.Liu and A.albus P.Y.Liu.Enzyme activities in Moye14 Amorphaphollus sp.at seedling stage and rooting period were similar and stronger than that at the tuber formation stage.There was no change of zymogram of peroxides enzymes in 4 species at the 3 stages;Enzyme activity was the strongest at tuber formation stage,the next was rooting period,that was the weakest at seedling stage,but A.xiei P.Y.was just opposite.