克百威及其代谢产物对小鼠DNA损伤的研究

采用微核试验和单细胞凝胶电泳技术研究了克百威及其4种主要代谢物的细胞及遗传毒性，对每种受试物设立3个不同剂量组，微核试验以小鼠活体给药后分离骨髓淋巴细胞，单细胞凝胶电泳试验采用分离外周血细胞后受试物处理的方法进行。结果表明，克百威、呋喃酚在两种方法中均显示阴性结果，可认为无明显细胞及分子毒性；3-羟基呋喃丹和亚硝基呋喃丹均表现明显的细胞毒性，而3-酮基呋喃丹虽无明显微核效应，却可引起细胞强烈的DNA单链断裂，后3种化合物可能具有对机体的毒性，需要进一步确定其毒害机制。

Damage of Carbofuran and Its Metabolites on DNA

Micronucleus(MN)assay and single -cell gel electrophoresis(SCGE)assay were employed to test the cytotoxic and genotoxic effects of carbofuran and its main metabolites.The animals we re exposed to these chemicals by intr operitoneal injection at different dosage in MNtest,and isolated peripheral blood lymphocytes w ere evaluated by SCGEafter a 1-h trea tment.We found that carbofuran and carbofuran -phenol s howed negative results,and hence have no obvious cell toxicity;while 3-hydroxycarbofuran and nitrosocarbofuran were both positive,3-ketocarbofuran did not induce MN formation but caused signific ant DNA migration in SCGE test.This may be suggested that3-ketocarbofuran,3-hydroxycarbofuran and nitrosocarbofuran be pote ntial mutagesis and further research be needed.