铁皮石斛原球茎诱导与增殖研究

以铁皮石斛嫩茎为外植体，采用幼嫩茎段→原球茎诱导→原球茎增殖途径，通过单因子、双因子以及正交试验诱导原球茎。研究结果表明，外植体灭菌条件为：70%酒精浸泡3s后，0.1%升汞溶液中浸泡8min，诱导铁皮石斛原球茎最佳培养基为MS＋6-BA0.5mg/L+NAA0.5mg/L+ 2,4-D1.0 mg/L，原球茎增殖最佳培养基为MS＋6-BA3.0mg/L+NAA0.3mg/L。

Study on PLB Induction and propagation of Dendrobium officinale

The explants in this paper,an experiments was carried with the bud of current year after certain treatment, followed the approach： stem→ PLB inducing→ PLB proliferating. Adopted single-factor, double-factor and orthogonal design methods, and studied the effects of the different treatments and sterilization time on the induction of PLB （protocorm-like body）. The results were as following： The best sterilizing method for stem was disinfected with 70% alcohol for 3s, and then treated with 0.1% HgCl2 for 8 min. The formula of the optimal culture medium for PLB induction was the MS＋6-BA0.5 mg/L ＋ NAA 0.5 mg/L ＋ 2,4-D1.0 mg/L. The best medium for PLB proliferation was MS＋6-BA3.0mg/L＋ NAA0.3mg/L.