黑龙江省中国林蛙(Rana chensinensis)群体遗传多样性及区域分化的初步研究(英文)

本文运用随机扩增多态DNA (RAPD)技术对黑龙江省8个地区野外采集的中国林蛙(Rana c hensinensis)个体进行群体遗传多样性及区域分化的初步研究。实验筛选出的10个RAPD引物 共扩增出78条DNA多态片段。用NJ（Neighbor-Joining）方法分析遗传距离并构建系统树。 结果表明，中国林蛙(R. chensinensis)在8个不同采样地区已经出现明显的区域分化，且不 同地区的中国林蛙之间存在地理距离和遗传距离不相吻合。根据地理、地质学资料初步推论 ，黑龙江省中国林蛙(R. chensinensis)的中心发源区很可能位于松花江和黑龙江两江下游 的丘陵地区。并主要依靠松花江水系从三江平原进入松嫩平原，在方正地区形成向西扩散过 程中的次中心发源区。

Population genetic diversity and regional differentiation of Chinese forest frog s (Rana chensinensis) in Heilongjiang Province

The Random Amplified Polymorphism DNA (RAPD) markers were used to stu dy the intra-specific diversity and regional differentiation of the Chinese fore st frogs (Rana chensinensis), which were sampled from the fields of 8 regions in Heilongjiang Province. Totally 78 polymorphic DNA loci were amplified by 10 RAP D primers. By genetic distance analysis and phylogenetic tree reconstruction wit h the Neighbor-Joining (NJ) method the results showed that the populations of Ch inese forest frogs distributed in 8 regions existed great differentiation (Avera ge Fst=0.347, SD=0.235) while there was the paradox between geographic distances and genetic distances. Based on geographic and geological data, a hypothesis wa s posed that it is very possible that the hilly lands in the downstream of the S onghua River and the Heilong River were the center of the origin of the Chinese forest frogs in Heilongjiang Province. And mainly through the Songhua River syst em, the Chinese forest frogs dispersed into the Songneng Plain from the Sanjiang Plain whereas the Fangzheng region became a sub-center for the western dispersi on.