| SCAR分子标记在甘蓝型油菜S单倍型分类中的应用 |
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| 引用本文： | 刘瀛,唐维.SCAR分子标记在甘蓝型油菜S单倍型分类中的应用[J].现代农业科技,2010(1):29-30. |
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| 作者姓名： | 刘瀛 唐维 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,湖北武汉,430070 |
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| 摘 要： | 根据克隆到的ISLGa片段与ISLGb片段核苷酸序列之间的差异,发展了1个SCAR分子标记ISLGb(ISLGb-L+PS15),其引物只在具有ISLGb基因型的3个材料中扩增,并且均得到了大小为1265bp的单一亮带。通过与已有的相关分子标记整合得到了1套SCAR分子标记体系。包括2个多重PCR分子标记:①ISLG(PS5+PS15)和SP110(SP11-L+SP11-R')用于鉴别ISLG和IISP11b;②ISLGb(ISLGb-L+PS15)与SP11a(SP11-L+SP11-R)用于鉴别ISLGb和IISP11。还包括1个单PCR分子标记:SRKa(S40-5+S60-2)用于鉴别IISRKa。
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| 关 键 词： | SCAR分子标记 甘蓝型油菜 S单倍型 分类 |
Application of SCAR Molecular Marker Technology for Classifying S Haplotype in Brassica napus |
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| Authors: | LIU Ying TANG Wei |
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| Institution: | LIU Ying TANG Wei (National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan Hubei 430070) |
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| Abstract: | According to differences between the nucleotide sequences of two fragments,ISLGa and ISLGb,a SCAR molecular marker named as ISLGb (ISLGb-L+PS15) was developed and only amplified a band of 1 265 bp in three ISLGb genotype materials.After integrating the relevant molecular markers,a set of SCAR molecular markers was acquired,including two multiplex PCR molecular markers:①ISLG (PS5+PS15) and SP110(SP11-L+ SP11-R’) were used to identify ISLG and IISP11b;②ISLGb(ISLGb-L+PS15) and SP11a(SP11-L+SP11-R) were used to identify ISLGb and IISP11.Meanwhile,a single PCR molecular marker,SRKa (S40-5+S60-2) was used to identify IISRKa. |
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| Keywords: | SCAR molecular marker Brassica napus S haplotype classification |
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