一个新的水稻黄绿叶突变体的遗传分析与基因定位

通过化学诱变获得一份稳定遗传的水稻黄绿叶突变体D83。该突变体苗期植株呈黄绿色，分蘖期开始逐渐转为淡绿色。与野生型相比，突变体苗期叶绿素a、叶绿素b和类胡萝卜素含量分别下降45.03%、53.93%和39.56%，成熟期每穗着粒数减少9.45%，千粒重下降10.76%。对D83与正常绿色品种杂交F₁、F₂代的遗传分析表明，D83的突变性状由一对隐性核基因控制。以D83/浙福802 F₂代作定位群体，应用分子标记将D83所携带的突变基因定位于水稻第2染色体短臂的SSR标记RM110附近，InDel标记Ch2-27和Ch2-32之间，该基因与这2个InDel标记的遗传距离分别为1.2 cM和2.3 cM。认为D83所携带的突变基因是一个新的水稻黄绿叶突变基因，暂命名为chl13(t)。

Genetic Analysis and Gene Mapping of a Novel Yellow-Green Leaf Mutant in Rice

An inherited stably yellow-green leaf mutant D83 was isolated by chemistry mutagenesis. Its whole plant exhibited yellow-green character at the seedling stage, and gradually changed into pea green from the tillering stage. Compared with its wild-type parent 10079, at the seedling stage, the contents of chlorophyll a, chlorophyll b and carotenoid decreased by 45.03%, 53.93% and 39.56%, at the maturation period, the number of spikelets per panicle reduced by 9.45%, the 1000-grain weight by decreased 10.76%, respectively. Genetic analysis of F₁ and F₂ generations of D83 crossed with green rice varieties showed that the yellow-green leaf character was controlled by one pair of recessive nuclear genes. Genetic mapping of the mutant gene was conducted by using molecular marker and F₂ mapping population of D83/Zhefu802. The results suggested that the mutant gene of D83 was mapped on the short arm of rice chromosome 2, nearby SSR marker RM110, between InDel markers Ch2-27 and Ch2-32. The genetic distances from the target gene to two InDel markers were 1.2 cM and 2.3 cM, respectively. The mutant gene of D83 was considered to be a new yellow-green leaf gene in rice and designated tentatively as chl13(t).