文心兰的茎尖及花梗组织培养和快速繁殖

文心兰茎尖和花梗用N6＋6－BA2.0mg/L培养基，花梗分化芽，茎尖同时分化芽和原球茎。6－BA低浓度促进分化芽，高浓度促进分化原球茎。1／2N6 6-BA0.2mg/L NAA0.2mg/L 香蕉汁100g/L培养基对继代增殖和壮苗生根效率均好。在此继代增殖培养基中接入原球茎则增殖生成近等量的原球茎和芽；接入芽则增殖大量的芽，产生少量的原球茎。香蕉汗有促进增殖和壮苗的作用。

In Vitro Propagation of Oncidium by Bud and Inflorescence Culture

A protocol was developed for in vitro propagation of Oncidium by culture of bud and inflorescence.The culture was initiated on N 6 medium containing 2.0mg/L 6 BA.The bud was placed on the above medium,shoots and protocorm like bodies were induced,inflorescence was placed on the above medium,only shoots were induced.Multiplication and in vitro rooting of shoots were achieved on 1/2 N 6 medium supplemented with 0.2mg/L 6 BA,0.2mg/L NAA and 100g/L banana juice,when subculture on the above medium,the shoots only produced shoots and developed into plantlet,the protocorm like bodies produced protocorm like bodies and shoots.