| 催化动力学直接光度法测定微量对苯二酚 |
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| 引用本文： | 黄金英,林新华,李清禄.催化动力学直接光度法测定微量对苯二酚[J].福建农林大学学报(自然科学版),1994(4). |
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| 作者姓名： | 黄金英 林新华 李清禄 |
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| 作者单位： | 福建农业大学基础部，福建医学院基础部 |
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| 摘 要： | 研究了对苯二酚（ＱＨ２）抑制脲酶催化尿素水解反应及其动力学条件．并以尿素与对－二甲基氨基苯甲醛显色反应为指示反应，建立了动力学光度法测定脲酶催化抑制剂ＱＨ２的新方法．其灵敏度为４．０×１０－１０μｇ／ｍＬ，测定范围为０－０．１２μｇ／ｍＬ，操作简便、快速．该法用于测定乙醇溶液中微量的ＱＨ２，获得满意结果．
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| 关 键 词： | 动力学光度法 酶催化反应 脲酶抑制剂 对苯二酚 |
Direct Determination of Trace Hydroquinone by Catalytic Kinetic Spectrophotometry |
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| Huang Jinying, Lin Xinhua, Li Qinglu.Direct Determination of Trace Hydroquinone by Catalytic Kinetic Spectrophotometry[J].Journal of Fujian Agricultural and Forestry University,1994(4). |
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| Authors: | Huang Jinying Lin Xinhua Li Qinglu |
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| Abstract: | Trace urease-inhibitor hydroquinone (QH2) can inhibit urea enzymatic hydrolytic reaction, and its kinetic condition is proposed. A new method for the direct determination of trace hydroquinone is obtained by urea and P-dimethylaminobenzaldehyde devoloping action spectrophotomety. The sensitivity of the method is 4.0×10-10μg/mL, the range of determination is 0-0.12 μg/mL for hydroquinone. The method was simple and rapid,and was applied to the determination of trace hydroquinone in high purity alcohol with satisfactory results. |
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| Keywords: | kinetic spectrophotometry enzymatic reaction urease-inhibitor hydroquinone |
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