大鼠胫前肌亚体内基因表达与酶组化染色相关性研究

[目的]研究大鼠胫前肌亚体内不同肌纤维型的分布情况,并探讨RT-PCR方法与酶组织化学染色在定量研究方面的相关性。[方法]应用RT-PCR技术对大鼠胫前肌亚体内4种肌纤维型的表达进行研究,同时与琥珀酸脱氢酶(SDH)组织化学染色进行比较。[结果]2种方法的结果均表明4种肌纤维在胫前肌的前后亚体内表达量存在较明显的差异。前后亚体内肌球蛋白重链异构体相对含量均为Ⅱb>Ⅱa>Ⅰ>Ⅱx,而其中前亚体又以Ⅱb和Ⅱa型相对含量多于后亚体Ⅱb和Ⅱa型;前亚体Ⅰ与Ⅱx型相对含量少于后亚体Ⅰ与Ⅱx型。[结论]RT-PCR方法与骨骼肌肌纤维琥珀酸脱氢酶组织化学染色方法在定性定量研究方面具有相对的一致性。

Study on Gene Expression in Rat's Tibialis Anterior Muscle Subvolumes and Its Correlation with Enzyme Histochemistry Stain

[Objective] The aim was to study the distribution of different muscle fiber types of tibialis anterior muscle subvolumes in rat and discuss the correlation between the RT-PCR technique and the enzyme histochemistry stain on the aspect of quantitative study.[Method] The expression of 4 muscle fiber types of tibialis anterior muscle subvolumes in rat were studied by RT-PCR technique,which was compared with that by succinate dehydrogenase(SDH) histochemistry stain.[Result] The results of using the 2 methods all showed that there was significant difference on the expression among the 4 muscle fiber types in anterior and posterior subvolumes of tibialis anterior muscle.The relative content of myosin heavy chain isoform in anterior and posterior subvolumes was all Ⅱb>Ⅱa>Ⅰ>Ⅱx and among them,the relative content of Ⅱb and Ⅱa types in anterior subvolume were more than that in posterior subvolume and that of Ⅰ and Ⅱx types in the former was less than that in the latter.[Conclusion] The RT-PCR technique and muscle fiber SDH histochemistry stain had relative consistency on the aspects of qualitative and quantitative study.