| 几种东北果树DNA提取及RAPD鉴定 |
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| 引用本文： | 路文鹏,李昌禹,康玉荣,宋润刚,李晓红,赵淑兰,沈育杰.几种东北果树DNA提取及RAPD鉴定[J].特产研究,2006,28(4):26-28. |
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| 作者姓名： | 路文鹏 李昌禹 康玉荣 宋润刚 李晓红 赵淑兰 沈育杰 |
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| 作者单位： | 1. 中国农业科学院特产研究所,吉林,吉林132109
2. 吉林市农业科学院,吉林,吉林,132101 |
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| 摘 要： | 以软枣猕猴桃、黑加仑、越橘、梨、李、杏、小苹果和山定子等果树冷冻幼叶为试材,采用修改的CTAB法提取基因组DNA。利用CTAB提取液提取幼叶组织3次,以第2次提取的DNA产率和质量最好;第3次的质量最好,但产率下降。用第2次和第3次提取的DNA直接用于RAPD分析,重复2次,带型稳定。
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| 关 键 词： | 东北果树 基因组DNA RAPD鉴定 |
| 文章编号： | 1001-4721（2006）04-0026-03 |
| 收稿时间： | 2006-05-18 |
| 修稿时间： | 2006年5月18日 |
Extraction of Genome DNA of Some Kinds of Fruit Trees and Identification of RAPD |
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| LU Wen-peng,LI Chang-yu,KANG Yu-rong,SONG Run-gang,LI Xiao-hong,ZHAO Shu-lan,SHEN Yu-jie.Extraction of Genome DNA of Some Kinds of Fruit Trees and Identification of RAPD[J].Special Wild Economic Animal and Plant Research,2006,28(4):26-28. |
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| Authors: | LU Wen-peng LI Chang-yu KANG Yu-rong SONG Run-gang LI Xiao-hong ZHAO Shu-lan SHEN Yu-jie |
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| Institution: | 1.Institute of Special Economic Animal and Plant of Science, CAAS. Jilin 132109 , China; 2.The Institute of Agricultural Sciences in Jilin City. jilin 132101, China |
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| Abstract: | Extraction of genome DNA of seven kinds of fruit trees in the north-east of China via amended CTAB method from young frozen leaves as materials,the splitting leaf tissue was extracted for three times by CTAB buffer.And the result showed that the quality of DNA of the third time was best among the three times while the yield and quality of DNA of the second time were better than other two and the DNA of the second and third time were used for analyses of RAPD PCR directly which bands were stable for two repeats. |
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| Keywords: | Fruit tree in the northeast of China genome DNA identification of RAPD |
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