小麦耐盐相关基因TaSTK的克隆

利用RACE方法，从小麦耐盐突变体RH8706-49中扩增获得小麦丝氨酸/苏氨酸蛋白激酶（Triticum aestivum serine-threonine protein kinase）基因TaSTK的全长cDNA序列，含1 958 bp，其中开放阅读框1 431 bp，编码476个氨基酸。其编码区基因组DNA全长为4 095 bp，含5个外显子。该基因的全长cDNA序列及基因组序列均已提交GenBank数据库（登录号：DQ103756和DQ341377）。经过NCBI比对发现该基因的氨基酸序列与丝氨酸/苏氨酸蛋白激酶、酪氨酸蛋白激酶、蛋白激酶都具有较高的同源性。Northern杂交检测表明，TaSTK在小麦中属于盐诱导增强型基因，并且在耐盐材料RH8706-49中诱导增强程度高于敏盐材料H8706-34。TaSTK的杂交信号非常弱，表明TaSTK在小麦幼苗的叶片组织中属于低表达的基因类型。

Cloning of Salt-Tolerance Related Gene TaSTK in Triticum aestivum

The full-length cDNA sequence of TaSTK gene contained 1 958 bp was obtained from the salt-resistant wheat(Triticum aestivum) mutant RH8706-49 by RACE method.The coding sequence was 1 431 bp and by which 476 amino acids were encoded.The genome sequence of coding location contained 4 095 bp and 5 extrons.The cDNA sequence and genome sequence of TaSTK gene were submitted to GenBank (Accession No.QD103756,DQ341377).According to the BLAST results,the peptide encoded by this gene showed high homology to serine/threonine protein kinases(S-TKc,SPS1),tyrosine kinase(TyrKc) and protein kinase(Pkinase).Northern blotting results showed that TaSTK was a salt-induced gene in wheat.Under salt stress,the expression of TaSTK was elevated more strongly in the salt-tolerant line RH8706-49 than in the salt-sensitive line H8706-34.At the same time,the hybridization signals were all very weak,indicating that TaSTK belongs to the low expression gene in the wheat seedling tissue.