| 转基因油菜的花药培养 |
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| 引用本文： | 董五辈,唐桂芬,兰尊海,李学宝. 转基因油菜的花药培养[J]. 郑州牧业工程高等专科学校学报, 1995, 0(4) |
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| 作者姓名： | 董五辈 唐桂芬 兰尊海 李学宝 |
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| 作者单位： | 郑州牧专(董五辈，唐桂芬，兰尊海)，华中师范大学生物系(李学宝) |
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| 基金项目： | 国家“863”计划资助项目 |
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| 摘 要： | 本研究通过花药培养技术获得了6个抗Km 20mg/L的单倍体苗,其中花药愈伤组织的最高诱导率是21.6%,最佳激素配比是:Miller+2,4—D 2.0mg/L+KT1.5mg/L+IAA 2.0 mg/L,愈伤组织的苗最高分化率是18.8%,最佳激素配比是:MS+KT2.0mg/L+IAA 0.2mp/L.
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| 关 键 词： | 转基因油菜 花药培养 诱导率 分化率 最佳激素配比 |
ANTHER CULTURE OF TRANSGENIC RAPE (Brassica napus L.) |
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| Dong Wubei etal. ANTHER CULTURE OF TRANSGENIC RAPE (Brassica napus L.)[J]. Journal of Zhengzhou College of Animal Husbandry Engineering, 1995, 0(4) |
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| Authors: | Dong Wubei etal |
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| Affiliation: | Dong Wubei etal |
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| Abstract: | In anther culture of transgenic rape plants, six haploid plantlets of being with km-resistahce ( km-r 20mg/L) were obtained. The callus inducing frequency was about 21.6% ( Miller+2, 4-D2.0mg/ L+KT1.5mg/ L+IAA2.0mg/ L) and the plant regeneration frequency was about 18.8% ( MS+KT2.0mg/ L+IAA0.2mg/ L) , respentively. |
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| Keywords: | transgenic rape anther culture inductor rate differentiation rate maximum hormone ratio |
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