桦木酸对地塞米松致氧化应激小鼠血清指标的影响

本试验旨在研究桦木酸(BA)对地塞米松(Dex)诱导氧化应激小鼠血清指标的影响。将40只健康雄性昆明小鼠随机分为5组,即对照(NC)组、Dex组、0.25 mg/kg BA组、0.50 mg/kg BA组、1.00 mg/kg BA组,每组8只。NC组和Dex组灌服1%的可溶性淀粉,其余各组灌服混悬于1%可溶性淀粉溶液中不同剂量的BA,连续灌服14 d后,除NC组注射生理盐水外,其余4组均腹腔注射25 mg/kg Dex诱导氧化应激模型。15 h后,眼眶采血,收集血清。检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(ALP)活性,总蛋白(TP)、白蛋白(ALB)、总胆固醇(TC)、甘油三酯(TG)、总胆红素(T-Bil)、钙离子(Ca~(2+))、细胞色素C(CytC)含量,总抗氧化能力(T-AOC)、抑制羟自由基能力、超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)和丙二醛(MDA)含量。结果显示:BA预处理后,能够显著或极显著降低Dex诱导氧化应激小鼠血清ALP活性及TG、T-Bil、CytC含量(P0.05或P0.01),且呈量效关系;低剂量BA(0.25、0.50 mg/kg)显著或极显著降低了血清MDA含量(P0.01或P0.05),但高剂量BA(1.00 mg/kg)反而极显著升高了其含量(P0.01);能够显著或极显著升高血清Ca2+和GSH含量、SOD的活性、T-AOC和抑制羟自由基能力(P0.05或P0.01),并呈量效关系;降低了血清ALT和AST活性,但影响不显著(P0.05),对血清TP和ALB含量无显著影响(P0.05)。由此可见,BA能够改善Dex引起的小鼠血清指标的变化,对Dex诱导的氧化损伤有预防性的保护作用。

Effects of Betulinic Acid on Serum Parameters of Oxidative Stressed Mice Induced by Dexamethasone

The objective of this research is to evaluate the effects of betulinic acid ( BA) on serum parameters of oxidative stressed mice induced by dexamethasone ( Dex) . Forty male Kunming mice were randomly divided into 5 groups: control ( NC ) group, Dex group, 0. 25 mg/kg BA group, 0. 50 mg/kg BA group and 1.00 mg/kg BA group, each group had 8 mice. Mice in control group and Dex group were orally administered 1% starch solution and the other groups were orally administered different doses of BA for 14 days. Except NC group, mice in the other groups were intraperitoneal injected Dex at dosage of 25 mg/kg to set up oxidative damage model. After 15 hours, blood was collected from eyes to prepare serum. The activities of alkaline phos?phatase ( ALP) , alanine aminotransferase ( ALT) and aspartate aminotransferase ( AST) , the contents of total protein ( TP) , albumin ( ALB) , total cholesterol ( TC) , triglyceride ( TG) and total bilirubin ( T?Bil) , calci?um ions ( Ca2+) and cytochrome c ( CytC) , total antioxidant capacity ( T?AOC) , inhabitation capacity of hy?droxyl free radical, superoxide dismutase ( SOD) activity, and the contents of glutathione( GSH) and malond?ialdehyde ( MDA) were determined. The results showed as follows:BA pretreatment could significantly reduce serum activity of ALP and contents of TG, T?Bil and CytC of oxidative stressed mice ( P<0.01 or P<0.05) , and the effects were dose depended; BA pretreatment could significantly decrease serum content of MDA in low dose (0.25 and 0.50 mg/kg) BA groups (P<0.05 or P<0.01), while could significantly increase the content in high dose (1.00 mg/kg) BA group (P<0.01); BA pretreatment could significantly increase serum Ca2+and GSH contents, SOD activity, T?AOC and inhibition capacity of hydroxyl free radical ( P<0.05 or P<0.01) , and the effects were dose depended; BA pretreatment could decrease serum ALT and AST activities, but the effects were not significant ( P>0. 05 ) , and serum contents of TP and ALB were not significantly changed ( P>0.05) . The results suggest that BA can effectively improve serum parameters of mice and shows preventive protection of oxidative stress induced by Dex.