全自动体外模拟瘤胃发酵系统:19 kPa以下排气压力不影响发酵特征

本试验旨在研究不同排气压力对体外模拟瘤胃发酵特征的影响。选用3只体重相近、装有永久性瘤胃瘘管的成年湘东黑山羊作为瘤胃液供体动物,利用全自动体外模拟瘤胃发酵系统,选用6种发酵底物进行48 h体外发酵,研究9、14和19 k Pa 3种排气压力对总产气量、甲烷产量、产气参数、干物质消失率、发酵液p H以及挥发性脂肪酸产量等发酵参数的影响。结果表明:发酵底物对体外模拟瘤胃发酵参数影响显著(P0.05);排气压力对体外模拟瘤胃发酵参数没有显著影响(P0.05)。由此可见,排气压力由9 k Pa增至19 k Pa对体外模拟瘤胃发酵特征无影响。

Automated Incubation System in Vitro: Venting Pressure Less Than 19 kPa did Not Affect Fermentation Characteristics

The present study was conducted to determine the effect of different venting pressure on in vitro fer-mentation characteristics. Rumen fluid was collected from three adult Xiaongdong black goats with similar weight and permanent rumen fistula, used automated incubation system in vitro, and six widely varied sub-strates were selected for 48 h in vitro fermentation, and three venting pressures (9, 14 and 19 kPa) were per-formed to investigate the effects of venting pressure on in vitro total gas production, methane production, in vitro dry matter disappearance, fermentation fluid pH and volatile fatty acid production et al. The results showed that fermentation substrate had significant effects on in vitro rumen fermentation parameters ( P<0.05);however, venting pressure had no significant effects on in vitro fermentation parameters ( P>0.05) . In conclu-sion, venting pressure increase from 9 kPa to 19 kPa do not affect fermentation characteristics.