丁酸梭菌CBM01的碳、氮源优化及其对胃肠道耐受性的研究

本试验旨在探究丁酸梭菌CBM01的最佳碳、氮源及其对胃肠道的耐受性。采用控制变量的方法,以梭菌增菌培养基为基础,对丁酸梭菌CBM01的碳、氮源进行优化,同时对其胃肠道耐受性进行研究。结果显示:1)丁酸梭菌CBM01的最佳碳源和最佳氮源分别为葡萄糖和蛋白胨;2)丁酸梭菌CBM01在人工胃液和人工肠液中作用3 h后,存活率分别为90.33%和92.09%;3)丁酸梭菌CBM 01可耐受的最高胆盐浓度为0.40%。由此可见,丁酸梭菌CBM 01对胃肠道环境具有良好的耐受性,是一株开发潜力巨大、应用前景广阔的益生菌。

Optimization of Carbon and Nitrogen Sources of Clostridium butyricum CBM01 and Its Tolerance to Gastrointerestinal Tract

This experiment was conducted to investigate the optimal carbon and nitrogen source of Clostridium butyricum CBM01 and its tolerance to gastrointestinal tract. The reinforced clostridial medium ( RCM ) was used to optimize the carbon and nitrogen sources of Clostridium butyricum CBM01 with the method of control?ling variables, and the tolerance of Clostridium butyricum CBM01 to gastrointestinal tract was also determined. The results showed as follows:1) the best carbon source was glucose and the best nitrogen source was peptone for Clostridium butyricum CBM01; 2) the survival rates of of Clostridium butyricum CBM01 in the artificial gastric juice and artificial intestinal juice for 3 h were at 90.33% and 92.09%, respectively;3) the highest tol?erable concentration of Clostridium butyricum CBM01 to bile salt was 0.40%. Altogether, as Clostridium bu?tyricum CBM01 exerts great characteristics to tolerate gastrointestinal environments it can be considered as an i?deal potential probiotic strain for further researching.