不同玉米脱水酒精糟及其可溶物含量饲料中添加非淀粉多糖酶对大菱鲆幼鱼生长性能、营养物质消化率及抗氧化能力的影响

本试验旨在研究不同玉米脱水酒精糟及其可溶物(DDGS)含量饲料中添加非淀粉多糖酶对大菱鲆幼鱼生长性能、营养物质消化率及抗氧化能力的影响。选用720尾初重为(13.00±0.01)g的大菱鲆幼鱼,随机分为8个组,每个组设3个重复,每个重复30尾鱼。D3、D6、D9、D12组为不加酶对照组,分别饲喂以玉米DDGS替代基础饲料中3%、6%、9%、12%鱼粉的试验饲料;D3+、D6+、D9+、D12+组为加酶试验组,分别饲喂以玉米DDGS替代基础饲料中3%、6%、9%、12%鱼粉并添加木聚糖酶(活性为120 163U/g,添加量为20g/t)和纤维素酶(活性为13 424U/g,添加量为300g/t)的试验饲料。试验期为9周。结果表明:大菱鲆幼鱼饲喂相同玉米DDGS含量的饲料时,加酶试验组的终末体重、增重率、特定生长率、饲料效率均高于不加酶对照组,但差异未达显著水平(P0.05)。各组大菱鲆幼鱼摄食率以及全鱼水分、粗蛋白质、粗脂肪含量无显著差异(P0.05),肥满度、肝体比和脏体比也无显著差异(P0.05)。大菱鲆幼鱼饲喂相同玉米DDGS含量的饲料时,加酶试验组的干物质和粗蛋白质表观消化率均高于不加酶对照组,其中D9+组的干物质表观消化率较D9组显著升高(P0.05),D3+组的粗蛋白质表观消化率较D3组显著升高(P0.05),D12+组的粗蛋白质表观消化率较D12组显著升高(P0.05)。大菱鲆幼鱼饲喂相同玉米DDGS含量的饲料时,加酶试验组大菱鲆幼鱼血清超氧化物歧化酶和谷胱甘肽过氧化物酶的活性均较不加酶对照组显著提高(P0.05),血清丙二醛的含量较不加酶对照组显著降低(P0.05)。由此得出,在不同玉米DDGS含量饲料中添加非淀粉多糖酶提高了大菱鲆幼鱼对饲料的利用效果。

Effects of Different Contents of Corn Distillers Dried Grains with Soluble Diets Supplemented with Non-Starch Polysaccharide Enzymes on Growth Performance,Nutrient Apparent Digestibility Coefficients and Anti-Oxidative Ability of Juvenile Turbot (Scophthalmus maximus L.)

This experiment was conducted to investigate the effects of different contents of corn distillers dried grains with soluble (DDGS) diets supplemented with non-starch polysaccharide enzymes on growth performance,nutrient apparent digestibility coefficients and anti-oxidative ability of juvenile turbot (Scophthalmus maximus L.).Seven hundred and twenty juvenile turbots with the initial body weight of (13.00±0.01) g were randomly assigned to eight groups with three replicates per group and thirty turbots per replicate.D3,D6,D9 and D12 groups were enzyme free control groups,and the fish in them were fed the experimental diets which used corn DDGS to replace 3%,6%,9% and 12% fish meal individually.D3+,D6+,D9+ and D12+ groups were adding enzyme experimental groups,and the fish in them were fed the experimental diets which used corn DDGS to replace 3%,6%,9% and 12% fish meal individually and added 20 g/t xylanase (120 163 U/g) and 300 g/t cellulase (13 424 U/g).The trial lasted for 9 weeks.The results showed that the final body weight,weight gain rate,specific growth rate and feed efficiency in adding enzyme experimental groups were significantly higher than those in enzyme free control groups when fish fed the same corn DDGS content diet,but the differences were not significant (P>0.05).Feed intake,the contents of moisture,crude protein and crude lipid of whole body,condition factor,hepatosomatic index and viscerosomatic index were not significantly affected by diets supplemented with non-starch polysaccharide enzymes (P>0.05).The apparent digestibility coefficient of dry matter and crude protein in adding enzyme experimental groups were higher than those in enzyme free control groups when fish fed the same corn DDGS content diet.Among them,the apparent digestibility coefficient of dry matter in D9+ group was significantly higher than that in D9 group (P<0.05),the apparent digestibility coefficient of crude protein in D3+ group was significantly higher than that in D3 group (P<0.05),and the apparent digestibility coefficient of crude protein in D12+ group was significantly higher than that in D12 group (P<0.05).When fish fed the same corn DDGS content diet,compared with the enzyme free control groups,the activities of serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in adding enzyme experimental groups were significantly increased (P<0.05),while the content of serum malonaldehyde (MDA) was significantly decreased (P<0.05).In conclusion,the utilization effects of different contents of corn DDGS diets for juvenile turbot are improved by adding non-starch polysaccharide enzymes.